Abstract: The detection and analysis of aroma components of 5 high-bush blueberry cultivars in Guizhou were carried out by headspace solid-phase microextraction and gas chromatography−mass spectrometry (HS−SPME/GC−MS). The results showed that the types and contents of aroma substances varied greatly among varieties, among which 'O'Neal', 'Misty', 'Duck', 'Sharpblue' and 'Bluerain' contained 33, 35, 37, 30 and 32 ingredients respectively. The main aroma substances of 'O′Neal' are esters. The main aroma substances in 'Misty' are alcohols and aldehydes. The main aroma substances in 'Duck' and 'Bluerain' are benzene rings substances and alcohols. The main aroma substances in 'Sharpblue' are benzene ring substances and ester substances. Among them, the total aroma content of 'O'Neal' is the highest, which is 167 321.90 ng/g, whereas that of 'Bluerain' is the lowest, only 25 973.22 ng/g.