香樟不同树龄和组织的端粒酶活性测定

Telomerase Activity Detection in Different Age and Tissue of Cinnamomum camphora

  • 摘要: 从江西乐安县“中国第一古樟林”、安福县古樟群落和南昌县黄马香樟基地选7株不同树龄的香樟,取不同叶位高度的新叶和老叶,以及8个不同组织,即种皮、茎干树皮、枝条树皮、老叶、嫩叶、叶芽、花芽和花。利用6条先导引物和1条反向引物进行端粒酶扩展引物的筛选,其中TS21和RP引物的扩展效果最好。对所取样品用TRAP法测定端粒酶活性,结果显示端粒酶活性随着树龄的增加呈现下降的趋势;不同叶位高度叶片的端粒酶活性随着高度的增加逐渐降低;新叶端粒酶活性高于老叶;叶芽和花芽中端粒酶活性较高,说明分化能力较强的组织具有较高的端粒酶活性。

     

    Abstract: This study from the 'China first ancient Cinnamomum camphora forset' in Lean County, the forest community in Anfu Country and Huangma C. camphora base in Nanchang County of Jiangxi Province, sampling 7 different age of camphor trees, new and old leaves of different height, and 8 different tissues (episperm, stem bark, branch bark, old leaves, new leaves, leaf bud, flower bud and flower). Six forward primers and 1 reverse primer were used to screen telomerase amplification primers, the TS21 and RP primers had the best effect. The telomerase activity of the samples was measured by TRAP method. The results showed that the telomerase activity decreased with the increase of age. Leaves telomerase activity of different height decreased with the increase of leaf height. The telomerase activity of new leaves was higher than that old leaves. Telomerase activity was higher in leaf buds and flower buds, indicating that tissues with strong differentiation ability had higher telomerase activity.

     

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