Abstract: In order to reveal the biological function of the Dof gene family in the whole genome of Daemonorops jenkinsiana, we used bioinformatics method to identify the whole genome of the Dof gene family of D. jenkinsiana, and systematically analyzed the molecular evolution relationship between the Dof gene family of D. jenkinsiana and other 9 species. The results showed that there were 25 Dof genes in the genome of D. jenkinsiana, subcellular localization predicted that they were all located in the nucleus, the transmembrane structure predicted that all of the Dof protein members of D. jenkinsiana did not have transmembrane structure; the Dof protein family of D. jenkinsiana was unstable hydrophilic protein, and the secondary structure was mainly composed of α−helix, random coil, the third-order structure is mainly composed of α−helix and β−turn with complex spatial conformational changes; the number of amino acids of Dof protein members in D. jenkinsiana is 184 to 586 AA, and the pI range is 4.71 to 9.57. According to the phylogenetic analysis, the Dof gene family of D. jenkinsiana is divided into 4 subgroups of classⅠ–classⅣ. Among them, class Ⅱ has the most members (8 members), accounting for 32% of the total; class Ⅳ (7 members), class Ⅰ (5 members) and class Ⅲ (5 members) are the second; in terms of group, class Ⅱ has the fastest differentiation speed and the farthest genetic relationship with the original ancestors. From the group, DjDof9 and DjDof10 in class Ⅱ differentiate the fastest, have the longest genetic relationship with the original ancestors, DjDof12 and DjDof24 differentiate slowly, and have relatively close genetic relationship with the original ancestors. The phylogenetic analysis of Dof genes between D. jenkinsiana and other 9 species showed that there was not close genetic relationship between D. jenkinsiana and Arabidopsis thaliana, and the phylogenetic analysis of Dof genes showed that D. jenkinsiana was closely related to Brassica napus and Triticum aestivum, and had high sequence homology, and the farthest relationship with cacao.