Abstract: In order to explore the response law of stump sprouting capacity to stubble height and its biomass regulation mechanism of Hippophae rhamnoides ssp. sinensis, field experiments were carried out with a combination of random and latin arrangement with a stubble height of 0, 10, 20 cm, stem without stumping as control. The difference of sprout number, sprout growth, biomass accumulation and allocation pattern with different stubble heights were tested. The traits of tillering capacity and biomass accumulation were compared. The results showed that the number of sprouts increased with the increase of stubble height, while the survival rate, growth increment and biomass first increased and then decreased. The number of sprouts is negatively correlated with survival rate, there is a negative correlation trend between growth and survival rate, and the growth amount has a positive correlation with biomass. Compared with the control, the biomass accumulation and allocation of roots and leaves of stumping increased, and the biomass accumulation and allocation of trunk and branches decreased. These results indicated that the interference of stumping can stimulate the germination of adventitious buds and dormant buds, increase the biomass accumulation and allocation of root and leaf to promote the bud growth, and form the germination pattern of different stubble height under the trade off of the sprouting number, survival rate and growth increment. Among them, the survival rate and growth capacity of sprouting branches of 10 cm stubble height were the largest, and the biomass accumulation capacity of 10 cm stubble height after 3 growth seasons was significantly higher than that of the control (without stumping).