高效降解巨龙竹白腐菌株筛选及降解机制研究

Screening and Preliminary Degradation Mechanism of White Rot Fungi Strain of Dendrocalamus sinicus

  • 摘要: 为了获得高效降解巨龙竹木质素菌株,采用固体发酵法和化学测定法筛选高效降解巨龙竹木质素的菌株,并通过形态学与分子系统学相结合方法鉴定菌株;采用主成分分析揭示存在的降解类型,运用紫外扫描法揭示木质素初步降解机制。结果表明:从30株白腐菌株中筛选出1株高效降解巨龙竹木质素的菌株SWFU000072 Cerrena zonata,该菌株对巨龙竹具有极强的选择性降解木质素作用;30株白腐菌共存在3种降解类型:选择性降解木质素、选择性降解综纤维素、强烈选择性降解综纤维素;3株白腐菌降解后的乙醇提取液在260 nm处无明显的吸收峰,表明木质素中芳香环结构遭到破坏。白腐菌通过破坏木质素中的芳香环结构完成高效降解巨龙竹木质素的降解机制。

     

    Abstract: In order to obtain high efficient degradation strains of Dendrocalamus sinicus, this study employed the solid fermentation method and chemical determination method to screen the strains that could degrade lignin efficiently. The strains were identified by combining morphology with molecular method. The degradation types were revealed by principal component analysis. Ultraviolet scanning was used to reveal the initial degradation mechanism of lignin. The results showed that the strain SWFU000072 of Cerrena zonata was selected from 30 white rot fungi to degrade lignin efficiently. The strain had strong selective degradation of lignin and caused little loss of synthetic cellulose. There were 3 degradation types in 30 strains of white rot fungi: selective degradation of lignin, selective degradation of synthetic cellulose, and intense selective degradation of synthetic cellulose. The ethanol extract of the 3 white rot fungi showed unobvious absorption peak at 260 nm, indicating that the aromatic ring structure in lignin was destroyed, thus it revealed that the white rot fungi could degrade lignin efficiently by destroying the aromatic ring structure in lignin.

     

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