Abstract: Taking urban agglomeration in central Yunnan as the research object, this paper selects 3 periods of Landsat remote sensing data of urban agglomeration in central Yunnan from 2000 to 2019, extracts 3 periods of green spatial information through object-oriented classification technology, using GIS spatial analysis method, transfer matrix model and landscape pattern index method, this paper conducts multi-scale study on the green spatial pattern of urban agglomerations, prefectures, cities and counties in central Yunnan from 2000 to 2019 from 3 aspects of overall dynamic characteristics, spatial transfer process and landscape pattern index change. The results indicate that the scale of urban agglomeration generally shows the trend of green space shrinking and non-green space expanding. The transfer of green space mainly occurs among forest land space, cultivated land space and shrub-grass space, and the degree of landscape fragmentation and landscape diversity in green space decreases. On the prefecture-city scale, 7 counties and cities in Kunming, Qujing and Honghe have the most serious reduction in green space, and there are different trends and differences in green space landscape pattern index. On the district-county scale, the districts and counties with the largest increase in forest land space and the largest decrease in cultivated land space and shrub-grass space are concentrated in the periphery of central Yunnan, while the districts and counties with the largest increase in non-green space are concentrated in central and southern Yunnan. The green space patches in peripheral counties of central Yunnan are getting simpler and simpler, and the landscape diversity in central and western Yunnan is decreasing. Multi-scale study not only helps to comprehensively analyze the overall level and dynamic characteristics of green space in various regions at different scales, but also helps governments to formulate corresponding green space protection and economic development measures at different scales, and is of great significance for promoting the construction of ecologically livable urban agglomeration in the plateau region of central Yunnan.