冯都煌, 刘会云, 张莉, 等. 促进油茶花粉萌发的营养物质配比研究[J]. 西南林业大学学报(自然科学), 2022, 42(1): 91–99 . DOI: 10.11929/j.swfu.202010060
引用本文: 冯都煌, 刘会云, 张莉, 等. 促进油茶花粉萌发的营养物质配比研究[J]. 西南林业大学学报(自然科学), 2022, 42(1): 91–99 . DOI: 10.11929/j.swfu.202010060
Feng Duhuang, Liu Huiyun, Zhang Li, Wu Xinlei, Ding Zuonan, Zhang Wenyuan, Tan Shu, Weng Xiaoting, Wang Jiawei. Study on the Ratio of Nutrients for Promoting Pollen Germination of Camellia oleifera[J]. Journal of Southwest Forestry University, 2022, 42(1): 91-99. DOI: 10.11929/j.swfu.202010060
Citation: Feng Duhuang, Liu Huiyun, Zhang Li, Wu Xinlei, Ding Zuonan, Zhang Wenyuan, Tan Shu, Weng Xiaoting, Wang Jiawei. Study on the Ratio of Nutrients for Promoting Pollen Germination of Camellia oleifera[J]. Journal of Southwest Forestry University, 2022, 42(1): 91-99. DOI: 10.11929/j.swfu.202010060

促进油茶花粉萌发的营养物质配比研究

Study on the Ratio of Nutrients for Promoting Pollen Germination of Camellia oleifera

  • 摘要: 为探究固体培养基中添加蔗糖、硼酸、尿素、CaCl2、KH2PO4、PEG−3350、维生素C、维生素B1等对油茶花粉萌发率及花粉管萌发长度的影响,以‘赣无2’、‘赣70’、‘长林166’等油茶品种的成熟花粉为试材,分别采用单因素和正交试验,筛选出促进油茶花粉萌发的基础培养基和促进油茶花粉萌发的最佳营养物质配比。结果表明:促进油茶花粉萌发的基础培养基为5 g/L琼脂+100 g/L蔗糖+0.075 g/L硼酸。KH2PO4、PEG−3350对油茶花粉萌发和花粉管伸长具有显著促进作用,而尿素、CaCl2仅具有一定促进作用但其效果不显著。维生素C和维生素B1对油茶花粉萌发率均存在一定的抑制作用,但对花粉管伸长具有一定的促进作用。对油茶花粉萌发效果最好的2种营养成分为40~120 mg/L的KH2PO4、50~200 mg/L的PEG−3350;促进油茶花粉萌发的最佳营养物质配比为基础培养基(5 g/L琼脂+100 g/L蔗糖+0.075 g/L硼酸)+60 mg/L的KH2PO4+150 mg/L的PEG−3350。

     

    Abstract: We study the effects of eight different nutrients maters including the sucrose, boric acid, urea, calcium chloride, potassium dihydrogen phosphate, PEG−3350, vitamin C and vitamin B1 on the pollen germination rate(PGR) and pollen germination length(PGL) of Camellia oleifera under the solid medium. The mature pollen of 'Ganwu2', 'Gan70', 'Changlin 166' were collected randomly, the single factor and complete orthogonal tests were used, to screen the sucrose-boric acid basic medium and other nutrients for promoting the pollen germination of C. oleifera, finally, the best nutrients composition could be determined. The result shows that the basic medium for pollen germination of C. oleifera was 5 g/L AGAR+100 g/L sucrose+0.075 g/L boric acid. KH2PO4 and PEG−3350 had obvious promoting effect on the pollen germination and pollen tube elongation of C. olerifera, while urea and calcium chloride without significant promoting effect. Vitamin C and Vitamin B1 had certain inhibitory effect on the pollen germination rate of C. oleifera, but they promoted the elongation of pollen tube. The best matters for promoting the pollen germination of C. oleifera were KH2PO4 (40–120 mg/L) and PEG−3350 (50–200 mg/L). The optimal nutrients composition for promoting the pollen germination of Camellia oleifera was as follows: The basic medium(5 g/L AGAR+100 g/L sucrose+0.075 g/L boric acid)+60 mg/L KH2PO4+150 mg/L PEG−3350.

     

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