Abstract: Two years of field experiments were conducted to investigate the wheat nitrogen(N) uptake, accumulation and N use efficiency in wheat and faba bean strip intercropping system. Four N levels(the N application rate for wheat at N₀, N₁, N₂, and N₃ levels was 0, 90, 180 and 270 kg/hm², respectively) and 2 planting patterns including mono-cropped wheat(MW) and wheat and faba bean strip intercropping(W//F) were designed, so as to further reveal the mechanism of high N use efficiency in W//F. The results showed that W//F only increased border row intercropping wheat(IW₁) shoot N content at jointing stage as compared to MW, and no difference in shoot N content between inner intercropping wheat and MW was found. However, the wheat spike N content was increased when wheat intercropped with faba bean under N₀, N₁ and N₂ levels, and the tendency was IW₁(border row wheat which close to the faba bean in a intercropping pattern) > IW₂(the second row of intercropping wheat which slightly far away from the faba bean in a intercropping pattern) > MW(mono-cropped wheat) = IW₃(the third row of intercropping wheat which far away from the faba bean in a intercropping pattern). Secondly, the more N was allocated to wheat spike when wheat intercropped with faba bean, intercropping significantly increased N uptake in wheat spike, especially at maturity, the N accumulation in IW₁ and IW₂ spikes was significantly higher than MW at 4 nitrogen levels(N₀, N₁, N₂, and N₃), with increases of 40.23%–21.84% and 31.78%–10.13%, respectively; the N allocation proportion of IW₁ and IW₂ spikes increased by 19.74%–9.87% and 37.09%–4.65%. Wheat N use efficiency in intercropping either row was higher than MW, and N use efficiency for IW₁, IW₂ and IW₃ increased by 17.26%, 18.20% and 19.52% in comparison with MW in 2 years experiments; the overall nitrogen harvest index showed IW₁ = IW₂ > IW₃ > MW. In conclusion, W//F seldom changed wheat N content in leaf, but increased wheat spike N content and improved N shift from stem and leaf to spike. Both border and inner row effect were help to increase wheat N uptake and utilization in intercropping system. Optimal N application rates to stimulate intercropping advantage of inner row could maximize intercropping saving N effect.