Abstract: The secondary metabolites in cell suspension cultures of Vaccinium dunalianum were analyzed by LC−MS technique, the relative content of each metabolites was determined using the area normalization method, resulting in the identification of 208 secondary metabolites including 79 phenolic acids, 70 flavonoids, 15 terpenoids, 12 proanthocyanidins, 12 phenylpropanoids, 11 alkaloids, and 9 others. Their relative contents were 33.244%, 23.167%, 19.786%, 15.138%, 1.401%, 2.200% and 5.064%, respectively. The phenolic acids were composed of 34 phenolic glycosides and 45 other phenolics, with a relative content of 22.072% and 11.172%, while the flavonoids were composed of 58 flavonoid glycosides and 12 other flavonoids, with a relative content of 13.765% and 9.402%, respectively. It is noted that 26 compounds with a relative content of more than 1% were the main components in the cell suspension cultures of V. dunalianum, whose total relative content reached to 72.180%. Among them, procyanidin A2 was the compound with the highest relative content(8.655%), followed by catechin(8.030%), maslinic acid(7.503%), 2–hydroxyoleanolic acid(7.318%), and dunalianoside B(3.705%). The results will provide the scientific basis for further elucidating the high-yield mechanism of caffeoyl arbutins in V. dunalianum.