Abstract: 7-year-old Dalbergia odorifera was taken as the research object to explore the effects of different amounts of calcium and film mulching on growth and heartwood formation of D.odorifera. Three calcium treatments with different gradients(50, 100 g/plant and 150 g/plant) were applied respectively in film mulching and no film mulching way. The results showed that compared with the control group, the tree height increment of Ca₁₀₀, F + Ca₅₀, F + Ca₁₀₀ and F + Ca₁₅₀ treatment was increased by 37.93 %–71.03 %. No significant difference was found in DBH increment between D.odorifera and the control group. The content of total flavonoids in each treatment increased, and when the same amount of calcium was applied, the content of total flavonoids in film mulching treatment was significantly higher than that in non film mulching treatment. Among them, the treatment with F + Ca₁₅₀ had the highest total flavonoids content, which was 4.10 times that of the control group. The contents of characteristic substances in heartwood such as Nerolidol, (1R,3E,7E,11R)-1,5,5,8-Tetramethyl-12-oxabicyclo9.1.0dodeca-3,7-dieneand Medicarpin were also increased, and Ca₅₀, F + Ca₅₀ and F + Ca₁₅₀ were the treatments with higher relative contents. The synergistic treatment of calcium application and film mulching was beneficial to the growth of height and the improvement of heartwood quality of D.odorifera, which could provide a new cultivation idea for artificial promotion of heartwood formation of D.odorifera.