Abstract: A microwave digestion-inductively coupled plasma mass spectrometry(ICP−MS) method was developed for the determination of 47 trace elements in grape seed extract by isotope, the method was validated by the reference material of celery component analysis. The results showed that linearity of the proposed method was good, the correlation coefficient(R) was 0.9959–0.9999, and the detection limit of trace elements was less than 0.1 μg/L. The relative standard deviation(RSD) was 0.1%–5.4% for 6 successive times and the recovery was 82.7%–112.8%. The contents of K, Ca, Mg, Na, Fe were the highest, the content of Al, Mn, Ni, Cu, B, Zn, Rb, Sr, Sn, Ba, Mo were relatively higher, and the contents of Pb, As, Hg, Cd, Cr and 15 rare earth elements were relatively low in 11 lots of grape seed extracts. Therefore, the method is specific and sensitiv.