Abstract: Four mixed substrates were designed using red soil, peat, perlite and coconut bran as substrate materials and red soil as control. The growth traits, chlorophyll content and biomass of Malania oleifera seedlings in different mixed substrates were measured, and the quality of container seedlings was comprehensively evaluated by correlation analysis, principal component analysis and membership function value. The results were as follows: there were significant differences in plant height, number of leaves, leaf area, chlorophyll content, the number of self-haustorium and biomass of M. oleifera seedlings among different mixed substrates treatments. The seedling height of T₁ – T₃ treatment were 33.27, 34.43 cm and 33.89 cm , respectively, which were significantly higher than that of T₄ treatment and CK. The leaves area of seedlings in T₁, T₂ and T₄ treatment were 10.79, 12.21 cm² and 11.88 cm², which were significantly larger than that of CK; there were significant differences in the number of leaves and self-haustorium between T₂, T₃ treatment and CK. The chlorophyll content in T₂ treatment was the highest, which was 7.75 × 10^-4 mg/g, significantly different from T₃ treatment and CK. Meanwhile, the aboveground, aboveground, whole plant fresh mass, dry mass and strong seedling index of four treatments were significantly higher than those of the control. There was very significant positive correlation among plant height, leaf number, leaf area and biomass indexes. The comprehensive evaluation value of different mixed substrates was T₂ treatment > T₃ treatment > T₁ treatment > T₄ treatment > CK treatment. M. oleifera seedlings show obvious growth advantages in T₂ treatment, which was the best mixed substrate for container seedlings of M. oleifera.