Abstract: From August 2 to 4, 2020(the flowering period of Artemisia ordosica), the daily variation of its three-dimensional space pollen transmission was monitored by using the gravity slide method, once every 3h, for 3 consecutive days. The results showed that the diurnal variation of pollen amount could be described by a univariate cubic function within 24 hours of the blooming period of A. ordosica. The pollen amount was the least from 02: 00 to 03: 00, and the most from 14: 30 to 15: 00, with unimodal and explosive characteristics; In terms of spatial change, the number of pollens at the upwind end of the local prevailing wind is significantly less than that at the downwind end, and the number in the south is the least, which is significantly less than that at the north of the downwind end, and the number at 3m is significantly less than that at 1m. Pollen amount was positively correlated with air temperature(P < 0.01), negatively correlated with relative humidity(P < 0.01), and positively correlated with wind speed. Path analysis showed that temperature was the dominant factor affecting pollen propagation, followed by wind speed and relative humidity. Therefore, creating a local small environment with relatively low wind speed, low air temperature and high humidity through ecological or biological measures will help reduce the spread and transmission of A. ordosica pollen.