Abstract: Four kinds of herbicides(high-efficiency flumepyrazim, benazolin , glufosinate and dropping acid glyphosate) were used to spray Ligustrum quihoui hedgerow seriously infected by Cuscuta japonica. After 1 d, 5 d and 10 d of application, the contents of soluble sugar(SS), sucrose(SU), starch(ST) and pyruvate(PA) and the activities of nitrate reductase(NR), superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) in stems of C. japonica and leaves of L. quihoui were determined respectively, to analyze the difference effects of herbicide types and application days on the physiological metabolism and antioxidant enzyme system between C. japonica and L. quihoui. The results show that herbicides types and application days had significant effects on the contents of SS, Su and ST in the 2 plants, as herbicides types effects on C. japonica was ST > SU> SS and days effects was SU > ST> SS, but on L. quihoui was SS > SU> ST similarly. Herbicides types and application days had significant effects on the PA contents and NR activity in the 2 plants, as herbicides effect on C. japonica was NR>PA while pesticide days was PA>NR, but the effects on L. quihoui was NR>PA only. Herbicides types and application days had significant effects on the SOD, POD and CAT activity in the 2 plants, with herbicides types effects on C. japonica was CAT>SOD> POD and application days effects was SOD>CAT>POD, but herbicides and days effects on L. quihoui was SOD>CAT>POD samely. The effects of herbicide types and application days on the physiological metabolism and antioxidant enzyme system of C. japonica were greater than those of L. quihoui. The 4 herbicides had control potential on C. japonica, such as high-performance flupirone was the fastest and glufosinate was the largest effect on C. japonica, but glufosinate had serious potential drug damage to L. quihoui.