Abstract: The U^*₁₂(4¹⁰) uniform design, which was deformed from the U^*₁₂(12¹⁰) was applied to implement seedling raising trial of maternal trees of seed collection, microwave radiation time and seed soaking with different concentration solution of exogenous hormones. The results showed that mean basal diameters(BDs) was 2.57–3.28 mm, seedling heights(SHs) and taproot lengths(TRLs) were 13.8–19.9 cm and 17.0–25.8 cm, the biomass of root(BOR), stem(BOS), leaf(BOL) and whole seedling(BOWS) were 0.31–0.83, 0.27–0.63, 0.60–1.40, 1.18–2.87 g/seedling, seedling quality index(SQI) was 0.17–0.38 of treatment combinations(TCs) for 274-day-old seedlings. There were significant differences of mentioned parameters between the TCs excepting of which stem biomass. The leaf was an important organ constituting the seedling biomass of Cyclobalanopsis glaucoides which accounting for about 1/2 of the whole seedling. The seedlings of the tree species belong to the type with high water content, which was constituted about 1/2 of the fresh seedling biomass. The maternal tree of seed collection was the dominant factor affecting the seedling growth and biomass accumulation of C. glaucoides. There were significant differences in seedling growth and biomass between maternal trees. The comprehensive quality of seedlings was the best with the TC of 1^# maternal tree seeds via microwave radiation for the 5S, and then soaked in 1.5 and 0.35 g/L GA₃ and IAA solution. The seedling growth and biomass accumulation of C. glaucoides are dominantly affected by the heredity, and its sound seedling cultivation needs to be carried out on the basis of selecting maternal trees of seed collection. Soaking seeds with exogenous hormones could promote the growth and biomass accumulation of C. glaucoides seedlings on the basis of improved varieties.