Abstract: Hainan Tropical Rainforest National Park which is the the hot spot and key area of global biodiversity conservation was taken as the research case. Based on the data as digital elevation model(DEM), soil, forest, population, economy, normalized difference vegetation index(NDVI) and rare endangered wildlife, the minimum cumulative resistance(MCR) model and gravity model were combined to identify major ecologicalsource, and on this basis, major ecological corridors in the national park were identified and constructed. The results showed that 13 major ecological sources were identified with the total area of 83300.78 hm², and 6 ecological corridors with a width of 600 m, a total length of 327.71 km, and a total area of 19294.05 hm² were constructed, including 3 mahor corridors and 3 general corridors. The ecological corridors constructed projects include natural forest tending and degraded forest restoration, artificial forest near naturalization transformation, water source protection, etc, and reconnected the areas isolated by highways in the study area, effectively reducing habitat fragmentation. This proposed method can provide a reference and landing point for future biodiversity conservation in national parks, clarify the direction of conservation, restoration and management work, and provide a reference for improving habitat connectivity and building a structural integrity of the regional landscape ecological pattern.