Abstract: With random route inspection method as the main method and sample plot method as the auxiliary method, a systematic investigation was conducted on the macrofungi under the forest in this area. In addition to random route inspection, 8 sample plots were selected. The findings revealed that there were 223 species of macrofungi in Gaoligongshan, belonging to 46 families and 86 generas. There are 77 kinds of edible, 21 kinds of medicinal fungi, 25 kinds of poisonous fungi, 15 kinds of food and medicine, and 80 kinds of fungi which we aren’t sure their applicatians. In this area, 1 species is vulnerable, 1 species is near threatened, 101 species are least concern, 8 species are endemic to China. The dominant families are Russulaceae, Polyporaceae, Cortinariaceae, Boletaceae, Agaricalcea, Mycenaceae. Russula, Lactarius, Amanita, Cortinarius, Mycena, Marasmius, Polyporus, Entoloma, Laccaria, Trametes, Lycoperdon, Hygrocybe, Microporus are the dominant genera. Differ from dominant genera in different places. The macrofungi with obvious temperate nature, and also had a certain relationship with tropical-subtropical macrofungal flora. It is similar to Daweishan in Yunnan.