Abstract: A greenhouse hydroponic simulation experiment was conducted to study the prometryn content in water at 0, 6, 12, 24, and 30 days under different concentrations of prometryn pollution in Vetiveria zizanioides, Acorus calamus, and their 2∶1 configuration. The findings revealed that in water polluted by different concentrations of prometryn, the removal rate of prometryn in the plant planting group was 58.60%–91.69% on the 30^th day, while in the control group (without planting plants), the removal rate of prometryn was 45.86%–57.48% on the 30^th day. The plant planting group was significantly higher than the control group. When the initial concentration of prometryn was <4 mg·L⁻¹, the removal efficiency of V. zizanioides(V) for prometryn pollution in water was the highest, reaching 88.4% on the 30^th day. When the initial concentration of prometryn was ≥ 4 mg· L⁻¹, the 2∶1 combination of V. zizanioides and A. calamus(2V-1A) had the highest removal efficiency of prometryn pollution in water, reaching 91.69% on the 30^th day. This study provided references for phytoremediation of water bodies polluted by prometryn at different concentrations.