Abstract: Based on the List of National Key Protected Wildlife and Plants Distributed in Yunnan Province by Prefecture and City(2021), a supplemental list of the distribution of national key protected terrestrial wildlife in Yunnan Province by using the Chinese Biological Species List(2023) as a framework was formed through extensive collection and collation of scientific information, citizen science data, combined with certain field surveys and interview surveys, the Genus−Family(G−F) diversity index was used to calculate the diversity of protected species in each prefecture and city for the new list. The species and the distribution pattern of national key protected terrestrial animal in Yunnan Province have been analyzed systematically. Results show that here are 403 species of terrestrial wildlife distributed in Yunnan Province and an increase of 18 species(4.68%) over the 2021 list(385 species). The G−F diversity index in Yunnan Province was 0.90, with a mean value of 0.78 ± 0.05 for each prefecture and city, the highest being Nujiang(0.90) and the lowest being Qujing(0.56). The similarity of national key protected terrestrial wildlife in Yunnan Province forms 6 regions, and the distribution of species diversity shows a trend of high in the southwest and low in the northeast in terms of longitude and latitude. This study can provide a reference for updating the list of national key protected terrestrial wildlife, as well as for the protection and management of such wildlife distributed in prefectures(cities) and counties(districts) of Yunnan Province.