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基于LC−MS的樟叶越桔愈伤组织次生代谢成分分析
LC-MS Analysis of chemical constituents in the callus of Vaccinium dunalianum
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摘要: 以樟叶越桔健康愈伤组织为研究对象，采用LC−MS联用技术，对樟叶越桔愈伤组织的甲醇提取物进行分析。结果表明：正离子模式下共检测出14类共281个化合物，以萜类、生物碱类、有机酸、杂环化合物、糖苷类和醇类为主，化合物数量分别为58、41、28、24、22和13个，相对含量分别为16.866%、13.711%、6.108%、10.138%、12.488%和17.513%；负离子模式下检测出13类共71个化合物，以生物碱类、有机酸、糖苷类、萜类和酯类为主，化合物数量分别为14、9、9、7和7个，相对含量分别为5.839%、72.653%、3.423%、2.062%和2.735%，其中丙酮酸的相对含量高达47.474%。研究结果可为进一步开展樟叶越桔愈伤组织中主要化学成分的生产调控等研究提供参考。Abstract: Analysis of chemical constituents in the callus of Vaccinium dunalianum was carried out with LC-MS method. The results showed that a total of 281 compounds divided into 14 categories were detected in the positive ion mode, which mainly including 58 terpenoids, 41 alkaloids, 28 organic acids, 24 heterocyclic compounds, 22 glycosides and 13 alcohols, with a relative content of 16.866%, 13.711%, 6.108%, 10.138%, 12.488% and 17.513%, respectively. A total of 71 compounds divided into 13 categories were detected in the negative ion mode, among which 14 alkaloids, 9 organic acids, 9 glycosides, 7 terpenoids and 7 esters were the main components with a relative content of 5.839%, 72.653%, 3.423%, 2.062% and 2.735%, respectively. Especially, the relative content of pyruvic acid was the highest (47.474%). The results will provide a basis for the further regulation of the major chemical constituents in the callus of V. dunalianum.
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