吴博宇, 鲁斌, 尹文行, 等. 产香云芝菌丝体的培养条件优化及其挥发性成分分析[J]. 西南林业大学学报(自然科学), 2024, 44(5): 1–8. DOI: 10.11929/j.swfu.202311019
引用本文: 吴博宇, 鲁斌, 尹文行, 等. 产香云芝菌丝体的培养条件优化及其挥发性成分分析[J]. 西南林业大学学报(自然科学), 2024, 44(5): 1–8. DOI: 10.11929/j.swfu.202311019
Wu Boyu, Lu Bin, Yin Wenhang, Xiao Weihong, Wang Wei, Yan Dong, Zhao Ping. Optimization of culture conditions and analysis of volatile components of a fragrant Trametes versicolor mycelium[J]. Journal of Southwest Forestry University. DOI: 10.11929/j.swfu.202311019
Citation: Wu Boyu, Lu Bin, Yin Wenhang, Xiao Weihong, Wang Wei, Yan Dong, Zhao Ping. Optimization of culture conditions and analysis of volatile components of a fragrant Trametes versicolor mycelium[J]. Journal of Southwest Forestry University. DOI: 10.11929/j.swfu.202311019

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产香云芝菌丝体的培养条件优化及其挥发性成分分析

Optimization of culture conditions and analysis of volatile components of a fragrant Trametes versicolor mycelium

  • 摘要: 为获得云芝菌丝体最佳产香培养条件和其挥发性成分,采用单因素试验法考察了不同碳源、氮源、pH值、培养基体积、培养温度、培养时间对云芝菌丝体产生挥发性成分的影响,通过响应面法优化其培养条件,并结合气相色谱−质谱联用法对云芝菌丝体挥发性成分进行分析。结果表明:培养基体积、培养时间、培养温度对云芝菌丝体挥发性成分具有显著影响,最佳培养条件为以蔗糖为碳源、不添加氮源、培养基体积120 mL、培养温度27.5 ℃、培养时间27 d,在此条件下验证试验结果与理论预测的误差为0.315%。从云芝菌丝体中共检测出6种挥发性成分,包括脂肪醇类3种、芳香族类1种、倍半萜烯类2种,其中1−表双环倍半水芹烯和(E)−菖蒲烯为主要成分,相对含量分别为29.96%和29.17%,为云芝菌丝体在微生物香料的进一步开发应用提供科学依据。

     

    Abstract: In order to obtain the optimal culture conditions and volatile components of Trametes versicolor mycelium, a single factor experimental method was used to study the effects of different carbon sources, nitrogen sources, pH values, medium volume, culture temperature, and culture time on the production of volatile components in T. versicolor mycelium. The culture conditions were further optimized by response surface methodology and the volatile components of T. versicolor mycelium were analyzed by gas chromatography-mass spectrometry. The results showed that the volume of culture medium, culture time, and culture temperature had a significant impact on the volatile components of T. versicolor mycelium. The optimal culture conditions were sucrose as the carbon source, no nitrogen source added, 120 mL of culture medium volume, 27.5 ℃ of culture temperature, and 27 days of culture time. Under these conditions, the error between the validation test results with the theoretical prediction is 0.315%. A total of 6 volatile components were detected from the mycelium of T. versicolor, including 3 alcohols, 1 aromatics, and 2 sesquiterpenes. Among them, 1-epibicyclosesquiphellandrene and (E)-calamenene were the main components, with relative contents of 29.96% and 29.17%, respectively. This provides a basis for the further development and application of T. versicolor mycelium in microbial fragrances.

     

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