郑洋群, 尹以杭, 余思廷, 等. 2种樟树叶片基质添加对牛樟芝三萜含量及抗氧化活性影响[J]. 西南林业大学学报(自然科学), 2024, 44(5): 1–9. DOI: 10.11929/j.swfu.202311026
引用本文: 郑洋群, 尹以杭, 余思廷, 等. 2种樟树叶片基质添加对牛樟芝三萜含量及抗氧化活性影响[J]. 西南林业大学学报(自然科学), 2024, 44(5): 1–9. DOI: 10.11929/j.swfu.202311026
Zheng Yangqun, Yin Yihang, Yu Siting, Zhao Xiaolei, ShiI Xiaolong, Zheng Yuan. Effects of Two Camphor Tree Leaf Substrates on the Triterpene Contents and Antioxidant Activities of Antrodia cinnamomea[J]. Journal of Southwest Forestry University. DOI: 10.11929/j.swfu.202311026
Citation: Zheng Yangqun, Yin Yihang, Yu Siting, Zhao Xiaolei, ShiI Xiaolong, Zheng Yuan. Effects of Two Camphor Tree Leaf Substrates on the Triterpene Contents and Antioxidant Activities of Antrodia cinnamomea[J]. Journal of Southwest Forestry University. DOI: 10.11929/j.swfu.202311026

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2种樟树叶片基质添加对牛樟芝三萜含量及抗氧化活性影响

Effects of Two Camphor Tree Leaf Substrates on the Triterpene Contents and Antioxidant Activities of Antrodia cinnamomea

  • 摘要: 比较分析在大米培养基中添加2种樟树不同浓度叶片匀浆对牛樟芝三萜含量及抗氧化活性的影响,筛选出最适的培养条件,为牛樟芝人工培养及樟属植物资源开发利用提供参考。以大米培养基为对照,分别添加0、0.5、1、2、3、4 g共6个浓度的牛樟和香樟叶片匀浆基质,测量了6种不同处理下牛樟芝的生长特性及三萜含量,并比较其对DPPH自由基清除能力和总还原能力,以评价牛樟芝的抗氧化活性。结果表明:不同浓度的牛樟和香樟叶片匀浆基质添加均能显著提高牛樟芝的生长和生物活性,各处理分别在9~27 d完成高峰生长。经过生长特性因子综合评价得出,在大米培养基中添加3 g香樟基质显著促进了牛樟芝的生长,较对照提高了4倍,为促进牛樟芝生长的最优处理;添加1 g牛樟基质显著提高了牛樟芝的三萜含量、DPPH清除能力及总还原能力,较对照分别提高了5.2、15.8和23倍,为提高牛樟芝三萜含量及抗氧化活性的最优处理。

     

    Abstract: Compare and analyze the effects of adding two different concentrations of camphor tree leaf homogenate substrates to rice culture medium on the triterpenoid content and antioxidant activity of Antrodia cinnamomea, select the most suitable culture conditions, and provide reference for the artificial cultivation of A. cinnamomea and the development and utilization. Using rice culture medium as a control treatment(CK), a total of 6 concentrations of 0(CK), 0.5, 1, 2, 3, and 4 g of Cinnamomum kanehirai and Cinnamomum camphora leaf homogenate matrices were added. The growth characteristics and triterpene contcentration of A. cinnamomea mycelia were measured under the 6 different treatments, and their free radical scavenging and total reducing abilities against 1,1-diphenyl-2-trinitrylhydrazine(DPPH) were compared to evaluate the antioxidant activity of A. cinnamomea. The results showed that adding different concentrations of C. kanehirai and C. camphora leaf homogenate matrix significantly improved the growth and biological activity of A. cinnamomea, and each treatment completed peak growth from 9 to 27 days. After comprehensive evaluation of growth characteristics factors, added 3 g of C. camphora substrate to rice culture medium significantly promoted the growth of A. cinnamomea, which was four times higher than the CK, which was the optimal treatment to promote the growth of A. cinnamomea; Addition 1 g of C. kanehirai matrix significantly increased the triterpene contcentration, DPPH scavenging ability, and total reduction ability of A. cinnamomea, which were 5.2, 15.8, and 23 times higher than the CK, respectively. This is the optimal treatment for improving the triterpenoid content and antioxidant activity of A. cinnamomea. This study provides a theoretical basis for the further large-scale cultivation and development and utilization of A. cinnamomea.

     

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