聂佳佳, 张爱莲, 张爽. 取食不同植物对舞毒蛾幼虫生长发育及生化酶的影响[J]. 西南林业大学学报(自然科学), 2024, 44(5): 1–7 . DOI: 10.11929/j.swfu.202312023
引用本文: 聂佳佳, 张爱莲, 张爽. 取食不同植物对舞毒蛾幼虫生长发育及生化酶的影响[J]. 西南林业大学学报(自然科学), 2024, 44(5): 1–7 . DOI: 10.11929/j.swfu.202312023
Nie Jiajia, Zhang Ailian, Zhang Shuang. Effects of feeding on different food plants on the growth, development and biochemical enzymes of Lymantria dispar[J]. Journal of Southwest Forestry University. DOI: 10.11929/j.swfu.202312023
Citation: Nie Jiajia, Zhang Ailian, Zhang Shuang. Effects of feeding on different food plants on the growth, development and biochemical enzymes of Lymantria dispar[J]. Journal of Southwest Forestry University. DOI: 10.11929/j.swfu.202312023

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取食不同植物对舞毒蛾幼虫生长发育及生化酶的影响

Effects of feeding on different food plants on the growth, development and biochemical enzymes of Lymantria dispar

  • 摘要: 以垂柳、白桦、雪松、水杉、华山松、元宝枫饲喂舞毒蛾幼虫,研究了不同食料植物对幼虫生长发育和6种酶活性及其基因表达量的影响。结果表明:取食雪松的2龄幼虫的存活率最高为55.32%,发育历期最短为5.41 d;取食垂柳和白桦的存活率分别为41.34%和35.71%,发育历期分别为6.40 d和6.66 d;取食元宝枫、华山松、水杉的幼虫存活率显著低于取食其他3种植物且不能完成整个生命史。幼虫取食不同植物后,体内的6种酶活性差异显著,幼虫取食垂柳后的CarE、AChE、POD和CAT活性显著高于取食雪松的,取食雪松个体的GSTs和SOD活性显著高于取食垂柳的个体。GST、SOD活性与幼虫的存活率和发育历期呈正相关;CarE、AChE、POD、CAT活性与幼虫的体质量、头宽和体长呈正相关。取食垂柳个体的LdAChELdCAT的相对表达量极显著高于取食雪松的个体,LdPOD的相对表达量显著高于取食雪松的个体,取食雪松个体的LdSOD表达量极显著高于取食垂柳的个体。舞毒蛾幼虫能够随着取食植物种类的不同调整其体内的生化酶活性及酶基因的表达水平,以满足其生长发育的需要。

     

    Abstract: Fed with Salix babylonica, Betula platyphylla, Cedrus deodara, Metasequoia glyptostroboides, Pinus armandii and Acer truncatum, the effects of different food plants on the growth and development of the Lymantria dispar larvae, the activities of six enzymes and their gene expression were studied. The results showed that the highest survival rate of the 2nd instar larvae fed on C. deodara was 55.32%, and the shortest development duration was 5.41 d; the survival rates of S. babylonica and B. platyphylla were 41.34% and 35.71%, and the development duration was 6.40 d and 6.66 d; and the larvae fed on A. truncatum, P. armandii and M. glyptostroboides were significantly lower than that fed on the other three plants and could not complete the whole life history. There was a significant difference in the activity of six enzymes of the larvae after feeding on different plants. The activities of CarE, AChE, POD and CAT of the larvae after feeding on S. babylonica were significantly higher than those feeding on C. deodara; and the activities of GSTs and SOD of larvae feeding on C. deodara were significantly higher than those feeding on S. babylonica. The activities of GST and SOD were positively correlated with the survival rate and developmental duration of the larvae. The activities of CarE, AChE, POD and CAT were positively correlated with the weight, head width and body length of the larvae. The relative expression levels of LdAChE and LdCAT of larvae feeding on S. babylonica were extremely significantly higher than those in C. deodara, and the relative expression of LdPOD was significantly higher than those in C. deodara, and the expression levels of LdSOD of larvae feeding on C. deodara were extremely significantly higher than those in S. babylonica. The larvae of Lymantria dispar could adjust the biochemical enzyme activity and the expression level of enzyme genes in their bodies with different plant species to meet the needs of their growth and development.

     

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