Abstract: Camellia oleifera is one of the important woody oil-producing plants in China. The quality of C. oleifera seed oil is one of the key criteria to evaluate the quality of C. oleifera. Pollination experiments were carried out on 4 main varieties of Camellia oleifera(Changlin 53, Eyou 102, Huajin and Huaxin) in Hubei Province. The C. oleifera seed oil of the harvested fruit of pollination combination was determined by gas chromatograph, focusing on the effects of different pollination combinations on the fatty acid content of C. oleifera seed oil. The results showed that oleic acid and linoleic acid contents in the 17 pollination combinations ranged from 78.60% to 84.00% and from 5.06% to 10.27%, respectively. The artificial pollination combinations with higher oleic acid content included 'Huajin' × 'Huaxin', 'Huajin' × 'Eyou 102', 'Huaxin' × 'Eyou 102', while the artificial pollination combinations with higher linoleic acid content included 'Eyou 102' self-pollination and 'Huaxin' × 'Changlin 53'. Planting 'Huajin' as the main variety, interplanting with 'Eyou 102' and 'Huaxin' as pollination varieties can yield C. oleifera seed oil with high oleic acid content; planting 'Eyou 102' alone or planting 'Huaxin' as the main variety, interplanting with 'Changlin 53' as pollination variety can yield C. oleifera seed oil with high linoleic acid content.