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云南樟基质添加对牛樟芝生长特性的影响
Effects of Cinnamomum glanduliferum matrix addition on the growth characteristics of Antrodia cinnamomea
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摘要: 为探讨云南樟基质添加对牛樟芝生长特性的影响，在马铃薯琼脂培养基上添加云南樟10种浓度（0.125～64 g/L）的嫩枝、嫩叶及其混合物，恒温培养30 d，分析其菌落直径、菌丝密度、菌落颜色、菌落长势、菌丝生长速度、生长指数、生物量、SOD活性、TT含量的差异。结果表明：在添加云南樟嫩枝条件下，当添加浓度为1～8 g/L时，牛樟芝的生长特性和生物量较优；添加浓度分别为2～4 g/L和2 g/L时，牛樟芝的SOD活性和TT含量显著优于对照。在添加云南樟嫩叶条件下，当添加浓度分别为1～4 g/L、1～16 g/L时，其生长特性和生物量较优；添加浓度为0.5～2 g/L时，其SOD活性和TT含量显著优于对照。在添加云南樟枝叶混合物条件下，当添加浓度分别为2～16、4～16、16～32 、8～32 g/L时，其生长特性、生物量、SOD活性和TT含量均保持在较优水平。可见，添加云南樟4 g/L嫩叶基质，对牛樟芝生长指标和生物量的促进效果最优，其菌落直径达83.40 mm，菌丝生长速度达2.78 mm/d，生物量达0.23 g；添加云南樟2 g/L嫩枝基质，显著提高了牛樟芝的SOD活性（392.06 U/g）；添加云南樟16 g/L枝叶混合基质，则显著促进了牛樟芝的总三萜含量（29.32 mg/g）。Abstract:ObjectiveTo explore the effect of Cinnamomum glanduliferum substrate addition on the growth and physiological and biochemical characteristics of Antrodia cinnamomea.MethodPotato agar medium (PDA) was supplemented with Ten concentrations (0.125-64 g/L) of C. glanduliferum branches, leaves and their mixtures and cultured at constant temperature for 30 days, and the differences in colony diameter, mycelial density, colony color, colony growth potential, mycelial growth rate, growth index, biomass, superoxide dismutase (SOD) activity, and total triterpene (TT) content were analyzed.ResultUnder the condition of adding C. glanduliferum branches in PDA medium, the growth characteristics and biomass of A. cinnamomea were higher when the addition concentration was 1-8 g/L, and the SOD activity and TT content of A. cinnamomea were significantly higher when the addition concentration was 2-4 g/L and 2 g/L, respectively; Under the condition of adding young leaves of C. glanduliferum to PDA medium, its growth characteristics and biomass were higher when the concentration was 1-4 g/L and 1-16 g/L, respectively. When the concentration was 0.5-2 g/L, its SOD activity and TT content were significantly higher than those of the control; The growth characteristics, biomass, SOD activity and TT content were kept at a high level when the addition concentration was 2-16 g/L, 4-16 g/L, 16-32 g/L and 8-32 g/L, respectively, when the branches and leaves mixture of C. glanduliferum was added to the PDA medium.ConclusionAdding 4 g/L of C. glanduliferum young leaf as the substrate has the best promoting effect on the growth indicators and biomass of A. cinnamomea, with a colony diameter of 83.40 mm, a mycelial growth rate of 2.78 mm/d, and a biomass of 0.23 g; Adding 2 g/L of C. glanduliferum branches matrix significantly increased the SOD activity of A. cinnamomea (392.06 U/g); The addition of 16 g/L mixed substrate of C. glanduliferum branches and leaves significantly promoted the total triterpene content of A. cinnamomea (29.32 mg/g). This study can provide theoretical and practical basis for the optimization of artificial cultivation conditions and large-scale cultivation of A. cinnamomea.
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