Abstract: In this study, a total of 10 specimens were collected on 2 separate occasions from Yongjing County in Gansu Province, and Minhe Hui and Tu Autonomous County in Haidong City, Qinghai Province. Morphological comparison confirms that all specimens exhibit the following characteristics: a triangular head with the head length slightly greater than the head width, smooth skin, black spots on the back, a light green longitudinal line extending from the tip of snout to vent, well-developed jaw glands, round eardrums with diameter larger than eye diameter, short and stout hind limbs, blunt and rounded toe tips, and fully webbed toes without notches. The morphological characteristics of the 10 specimens were similar to those of Pelophylax mongolius(Schmidt, 1925) from Tumd Right Banner, Baotou City, Inner Mongolia(type locality of P. mongolius). Phylogenetic analysis based on concatenated gene fragments of 16S rRNA, 12S rRNA, COI and Cytb showed that the ten specimens clustered together with P. mongolius with short genetic distance(p-distance = 1.9 %). We confirm that the ten specimens represent new distribution records for P. mongolius in Gansu and Qinghai Province.