毛卓佳, 易敏, 蒋润迪, 等. 诱导山苍子胚性愈伤组织的影响因素研究[J]. 西南林业大学学报(自然科学), 2024, 44(6): 1–10. DOI: 10.11929/j.swfu.202401061
引用本文: 毛卓佳, 易敏, 蒋润迪, 等. 诱导山苍子胚性愈伤组织的影响因素研究[J]. 西南林业大学学报(自然科学), 2024, 44(6): 1–10. DOI: 10.11929/j.swfu.202401061
Mao Zhuojia, Yi Min, Jiang Rundi, Chen Shangying, Liang Qingyu, Wu Zuming, Wang Zongde, Fan Guorong, Fu Dan, Wang Jiawei. Embryogenic Callus Induction and Somatic Embryogenesis of Litsea cubeba[J]. Journal of Southwest Forestry University. DOI: 10.11929/j.swfu.202401061
Citation: Mao Zhuojia, Yi Min, Jiang Rundi, Chen Shangying, Liang Qingyu, Wu Zuming, Wang Zongde, Fan Guorong, Fu Dan, Wang Jiawei. Embryogenic Callus Induction and Somatic Embryogenesis of Litsea cubeba[J]. Journal of Southwest Forestry University. DOI: 10.11929/j.swfu.202401061

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诱导山苍子胚性愈伤组织的影响因素研究

Embryogenic Callus Induction and Somatic Embryogenesis of Litsea cubeba

  • 摘要: 以山苍子优良单株的近成熟种胚为外植体,分析不同消毒方式、激素组合、碳源、种源对胚性愈伤组织诱导的影响,筛选出山苍子胚性愈伤组织形成的最佳诱导条件。结果表明:以75%乙醇消毒内果皮5 min,无菌水冲洗5次,污染率最低为0.00%;江西省南昌市为胚性愈伤组织诱导的最佳种源,诱导率达7.04%;添加外源激素1.0 mg/L 6-BA + 0.2 mg/L 2,4-D + 0.5 mg/L IAA的胚性愈伤组织诱导率最高,达17.50%。本研究成功诱导出山苍子胚性愈伤组织,并在培养基MS + 0.5 mg/L 6-BA + 0.1 mg/L 2,4-D + 0.25 mg/L IAA中实现胚性愈伤组织的增殖与体细胞胚的分化,进而实现了体胚的萌发,为建立高效稳定的山苍子体胚发生体系提供了重要研究材料和技术基础。

     

    Abstract: In order to screen out the best induction conditions for embryogenic calls formation of Litsea cubeba, nearly mature seed embryos of L. cubeba good single plants were used as explants to analyze the effects of different disinfection methods, hormone combinations, carbon source type and seed source type on the embryogenic calls induction. The results showed that: sterilizing the endocarp with 75% ethanol for 5 min and rinsing with sterile water for 5 times resulted in the lowest contamination rate of 0.00%; Nanchang City, Jiangxi Province, was the best seed source location for embryogenic calls induction, with an induction rate of 7.04%; and the addition of the exogenous hormone 1.0 mg/L 6-BA + 0.2 mg/L 2,4-D + 0.5 mg/L IAA had the highest rate of embryogenic calls, with a total induction rate of 17.50%. In this study, we successfully induced embryogenic calls of L. cubeba and achieved proliferation of embryogenic calls and differentiation of somatic embryos in medium MS + 0.5 mg/L 6-BA + 0.1 mg/L 2,4-D + 0.25 mg/L IAA, and then realized the germination of somatic embryos, which provided an important research material and technological basis for the establishment of a highly efficient and stable somatic embryogenic system of L. cubeba.

     

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