胡星, 胡纪龙, 张敏, 等. 外源NO对盐胁迫下八角金盘叶片生理特性及解剖结构的影响[J]. 西南林业大学学报(自然科学), 2024, 44(6): 1–10. DOI: 10.11929/j.swfu.202401067
引用本文: 胡星, 胡纪龙, 张敏, 等. 外源NO对盐胁迫下八角金盘叶片生理特性及解剖结构的影响[J]. 西南林业大学学报(自然科学), 2024, 44(6): 1–10. DOI: 10.11929/j.swfu.202401067
Hu Xing, Hu Jilong, Zhang Min, Liu Jiao, Huang Xiaoxia. Effects of Exogenous NO on the Physiological Characteristics and Anatomical Structure of Fatsia japonica Leaves under Salt Stress[J]. Journal of Southwest Forestry University. DOI: 10.11929/j.swfu.202401067
Citation: Hu Xing, Hu Jilong, Zhang Min, Liu Jiao, Huang Xiaoxia. Effects of Exogenous NO on the Physiological Characteristics and Anatomical Structure of Fatsia japonica Leaves under Salt Stress[J]. Journal of Southwest Forestry University. DOI: 10.11929/j.swfu.202401067

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外源NO对盐胁迫下八角金盘叶片生理特性及解剖结构的影响

Effects of Exogenous NO on the Physiological Characteristics and Anatomical Structure of Fatsia japonica Leaves under Salt Stress

  • 摘要: 为研究外源NO对盐胁迫下八角金盘叶片生理特性及解剖结构的影响,以半年生八角金盘实生苗为材料,采用盆栽试验,以1.2% NaCl溶液进行盐胁迫处理,外源喷施NO供体,共处理30 d,测定盐处理后0、15(轻度)、30 d(中度)的光合参数、生理指标并观测叶片解剖结构。结果表明:八角金盘可以通过提高抗氧化酶活性,积累Pro,增加上角质层和主脉厚度,适应轻度盐胁迫;而中度盐胁迫导致八角金盘叶片光合、RWC、光合色素和SS含量显著降低,MDA显著积累,叶片叶肉组织紊乱且厚度明显变薄。喷施外源NO可有效减缓盐胁迫下八角金盘叶片光合的降低,显著增加叶片RWC及光合色素、SS和SP的积累,提高CAT和APX活性,显著降低MDA;此外,外源NO还能通过降低上表皮和上角质层厚度,促进叶肉生长,增加栅海比,提高八角金盘抗盐性。总之,八角金盘具有一定的抗盐能力,而外施NO能够有效缓解中度盐胁迫对八角金盘叶片的伤害,增强其对盐胁迫的抵御能力。

     

    Abstract: To study the effects of exogenous nitric oxide(NO) on physiological characteristics and the anatomical structure of Fatsia japonica leaves under salt stress(NaCl). The half-year-old Fatsia japonica was treated with 1.2% NaCl solution by pot experiment, and exogenous NO donor(15 mL, 0.1 mmol/L Nitroprusside Sodium) was sprayed on the leaf surface for a total of 30 processing days. Photosynthetic parameters and physiological indexes were measured at 0 d, 15 d(mild) and 30 d(moderate) after salt treatment, and observed the anatomical structure of leaves. The results were as follows: Fatsia japonica was able to adapt to mild salt stress by increasing the activity of antioxidant enzymes, accumulating Pro, adding thickness to the cuticle and main veins. However, moderate salt stress significantly reduced the photosynthesis, RWC, photosynthetic pigment, and SS content in the leaves of Fatsia japonica, leading to a significant accumulation of MDA(malondialdehyde) and disorganized mesophyll tissue, with a marked thinning of leaf thickness. The application of exogenous NO(nitric oxide) effectively mitigated the reduction in photosynthesis under salt stress, significantly increased leaf RWC, and the accumulation of photosynthetic pigments, SS, and SP, enhanced CAT and APX activities, and markedly reduced MDA levels. In addition, exogenous NO also reduced the thickness of the upper epidermis and cuticle layer, promoted mesophyll growth, increased the ratio of palisade to spongy tissue, and enhanced the salt tolerance of Fatsia japonica. In summary, Fatsia japonica has a certain degree of salt tolerance, and the application of exogenous NO can effectively alleviate the damage caused by moderate salt stress to Fatsia japonica leaves and enhance its resistance to salt stress.

     

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