Abstract: Taking Dendrocalamus giganteus in Xinping County of Yunnan Province as the research object, Landsat 8 OLI image as the main data source, combined with the measured data of 35 plots, based on the sensitivity analysis of PROSAIL model parameters, the look-up table of leaf chlorophyll content(LCC) and canopy reflectance of D. giganteus was established. The chlorophyll index(CI) and its ratio(CI/G) to the greenness index(G) were selected to invert LCC, and then the canopy chlorophyll content(CCC) was obtained by combining the leaf area index(LAI).The results show that the R² for CI and CI/G inversion of LCC were 0.48 and 0.52, and RMSE was 11.09 µg/cm² and 8.23 µg/cm², respectively. The R² for CI and CI/G inversion of CCC were 0.53 and 0.64, and RMSE was 0.50 g/m² and 0.43 g/m², respectively. The research results can provide technical support for regional monitoring of D. giganteus.