袁宗胜. 促生细菌对毛竹生长和叶片酶活性及土壤细菌群落的影响[J]. 西南林业大学学报(自然科学), 2025, 45(4): 1–11. DOI: 10.11929/j.swfu.202405050
引用本文: 袁宗胜. 促生细菌对毛竹生长和叶片酶活性及土壤细菌群落的影响[J]. 西南林业大学学报(自然科学), 2025, 45(4): 1–11. DOI: 10.11929/j.swfu.202405050
YUAN Zongsheng. Effects of growth-promoting bacteria on growth, leaf enzyme activity and soil bacterial community of Phyllostachys edulis[J]. Journal of Southwest Forestry University. DOI: 10.11929/j.swfu.202405050
Citation: YUAN Zongsheng. Effects of growth-promoting bacteria on growth, leaf enzyme activity and soil bacterial community of Phyllostachys edulis[J]. Journal of Southwest Forestry University. DOI: 10.11929/j.swfu.202405050

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促生细菌对毛竹生长和叶片酶活性及土壤细菌群落的影响

Effects of growth-promoting bacteria on growth, leaf enzyme activity and soil bacterial community of Phyllostachys edulis

  • 摘要: 选用高效解磷解钾固氮活性的产气肠杆菌、解淀粉芽孢杆菌和乙酸钙不动杆菌(Acinetobacter calcoaceticus)菌株,采用三元二次回归正交旋转组合处理,对毛竹实生幼苗进行灌根施用处理后,测定毛竹株高、根长和叶片SPAD值、过氧化氢酶(CAT)活性、可溶性糖含量,选取最优组合根际、非根际土壤进行高通量测序。结果表明:部分促生细菌组合处理的毛竹株高、根长和叶片SPAD值显著增加,最大处理组高于对照组30.87%、29.96%、7.83%;叶片CAT活性、可溶性糖含量同样受到促生细菌影响,CAT活性和可溶性糖含量分别提高266.38%、1.46%。交互效应表明产气肠杆菌与解淀粉芽孢杆菌在低施用量(<40 mL)下效果更好,二者与乙酸钙不动杆菌的效果在高施加量(>40 mL)下得以体现。促生细菌处理显著影响毛竹根际与非根际土壤细菌群落丰度和组成,以芽孢杆菌属、类芽孢杆菌属和鞘氨醇单胞菌属为主要优势菌属,促生细菌处理的土壤样本细菌丰度占比高于对照组,促生细菌显著促进物质代谢和能量循环相关的功能表达丰度。促生细菌通过改变根际土壤微生物群落结构,增强物质循环与代谢,达到促进植物生长的目的。

     

    Abstract: Select strains of Enterobacter aerogenes, Bacillus amyloliquefaciens and Acinetobacter calcoaceticus with high phosphorus-solubilizing, potassium-solubilizing and nitrogen-fixing activity. Using ternary quadratic regression orthogonal rotation combination processing, after root irrigation was applied to Phyllostachys edulis seedlings, the plant height, root length, plant height, leaf SPAD value, catalase activity, and soluble sugar content of P. edulis were determined. The optimal combination and rhizosphere and non-rhizosphere soil bacterial community structure were selected for high-throughput sequencing. The results shows that: P. edulis plant height, root length and SPAD value all increased significantly(the maximum treatment group was 30.87%, 29.96%, 7.83% higher than the control group); The CAT activity and soluble sugar content of moso bamboo leaves were also affected by growth-promoting bacteria. The CAT activity and soluble sugar content increased by 266.38% and 1.46% respectively. The interaction effect showed that E. aerogenes and B. amyloliquefaciens were more effective at low application rates(< 40 mL), while the effect of both and A. calcoaceticus was reflected at high application rates(> 40 mL). Alpha and beta analyze showed that growth-promoting bacterial treatment significantly affected the abundance and composition of rhizosphere and non-rhizosphere soil bacterial communities. Bacillus, Paenibacillus and Sphingomonas are the main dominant bacterial genera. The proportion of bacterial abundance in soil samples treated with growth-promoting bacteria was higher than that in the control group. Growth-promoting bacteria can significantly promote the abundance of functional expression related to material metabolism and energy cycle.Growth promoting bacteria can enhance the material circulation and metabolism by changing the rhizosphere soil microbial community structure, and achieve the purpose of promoting plant growth.

     

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