张红玲, 宝元朵, 张静, 等. 添加不同培养基质对牛樟芝生长特性及总三萜积累的影响[J]. 西南林业大学学报（自然科学）, 2025, 45(3): 1–9. DOI: 10.11929/j.swfu.202407005
引用本文: 张红玲, 宝元朵, 张静, 等. 添加不同培养基质对牛樟芝生长特性及总三萜积累的影响[J]. 西南林业大学学报（自然科学）, 2025, 45(3): 1–9. DOI: 10.11929/j.swfu.202407005
shu
Zhang Hongling, Bao Yuanduo, Zhang Jing, Wu Ziqiang, Zhang Ying, Zheng Yuan. Effects of Different Cultivation Substrates on Growth Characteristics and Total Triterpenoid Accumulation of Taiwanofungus camphoratus[J]. Journal of Southwest Forestry University. DOI: 10.11929/j.swfu.202407005
Citation: Zhang Hongling, Bao Yuanduo, Zhang Jing, Wu Ziqiang, Zhang Ying, Zheng Yuan. Effects of Different Cultivation Substrates on Growth Characteristics and Total Triterpenoid Accumulation of Taiwanofungus camphoratus[J]. Journal of Southwest Forestry University. DOI: 10.11929/j.swfu.202407005
shu

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添加不同培养基质对牛樟芝生长特性及总三萜积累的影响

Effects of Different Cultivation Substrates on Growth Characteristics and Total Triterpenoid Accumulation of Taiwanofungus camphoratus

    摘要
  • 摘要: 在基础培养基（PDA）中分别添加不同浓度的肉桂和楠木的嫩枝、嫩叶及枝叶混合物作为牛樟芝的培养基质，研究不同培养基质对牛樟芝菌丝体生长特性及总三萜积累的影响。结果表明：在PDA培养基中添加肉桂和楠木基质，对牛樟芝生长、生物量和总三萜积累有明显促进效果。添加16～32 g/L肉桂嫩枝能显著促进牛樟芝菌丝体生长和总三萜积累，总三萜含量显著提高了142.67%～207.85%；添加4 g/L肉桂枝叶混合物和8 g/L楠木嫩叶基质分别显著提高了牛樟芝鲜质量（196.00%）和干质量（112.50%）。相比于添加嫩叶、枝叶混合基质，添加肉桂和楠木的嫩枝更能有效促进牛樟芝菌丝体生长，添加肉桂嫩枝和楠木枝叶混合物更能有效提高牛樟芝总三萜含量。添加肉桂基质对牛樟芝的生长特性、鲜质量和总三萜含量的促进效果更好，而添加楠木基质则对牛樟芝的干质量积累更有利。总体上，在PDA培养基中添加浓度为16～32 g/L肉桂嫩枝，对牛樟芝的生长特性和总三萜积累的促进效果最为显著。

     

    Abstract: In this study, the basic potato dextrose agar (PDA) medium with different concentrations of Cinnamomum cassia and Phoebe zhennan tender branches, tender leaves, and a mixture of branches and leaves, was used for Taiwanofungus camphoratus culture medium to explore its effect on the growth characteristics of T. camphoratus mycelium and total triterpenoid accumulation. The results showed that adding C. cassia and P. zhennan substrates in PDA medium significantly affected the growth, biomass and total triterpene accumulation of T. camphoratus. Regarding the effect of substrate concentration, adding 16-32 g·L−1 of C. cassia tender branches significantly promoted the mycelium growth and total triterpene accumulation of T. camphoratus, and the total triterpene content was significantly increased by 142.67-207.85% compared with the control. Additionally, 4 g·L−1 C. cassia branches and leaves mixture and 8 g·L−1 P. zhennan tender leaves substrates significantly increased the fresh weight and dry weight of T. camphoratus by 196.00% and 112.50%, respectively. Comparing the type of added substrate organs, it was found that compared with tender leaves and branches and leaves mixture substrate, the addition of C. cassia and P. zhennan tender branches effectively promoted the growth of T. camphoratus mycelium. Furthermore, C. cassia tender branches and P. zhennan branches and leaves mixture effectively improved the total triterpenoid content of T. camphoratus. When comparing the substrates added, it was observed that adding C. cassia substrate was more effective in promoting the growth characteristics, fresh weight and total triterpene content of T. camphoratus, while the P. zhennan substrate was more favorable for the dry weight accumulation of T. camphoratus. In conclusion, the results indicated that adding C. cassia tender branches at a concentration of 16-32 g·L−1 to the PDA medium had the optimal effect on promoting the growth characteristics of T. camphoratus and had the most significant effect on enhancing total triterpenoid accumulation.

     

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