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古茶树优势内生山茶炭疽菌次生代谢产物LC−MS/MS分析
LC-MS/MS analysis of secondary metabolites in the endophytic fungus Colletotrichum camellia from an ancient tea tree
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摘要: 采用LC−MS/MS联用技术，对“锦秀茶祖”古茶树叶内生真菌之山茶炭疽菌大米固体发酵物中的次生代谢产物进行分析。结果表明：共鉴定出
3309 种化合物，包括生物碱类796个、萜类540个、脂肪酸类475个、氨基酸/肽类436个、莽草酸−苯丙素类419个、聚酮类161个、碳水化合物156个、其他类衍生物326个，相对含量分别为24.746%、12.035%、25.739%、14.267%、10.792%、3.813%、2.366%、6.242%。相对含量大于0.5%的化合物共有35个，相对含量合计为37.420%。研究结果可为进一步挖掘山茶炭疽菌的活性次生代谢产物及其功能提供参考。Abstract: Analysis of the secondary metabolites in the rice solid fermentation of Colletotrichum camelliae, a dominant endophytic fungus isolated from the leaves of 'Jin Xiu Cha Zu' cultivated ancient tea tree Camellia taliensis (W. W. Smith) was carried out by using LC-MS/MS method. The results showed that a total of3309 compounds were identified, including 796 alkaloids, 540 terpenoids, 475 fatty acids, 436 amino acids and peptides, 419 shikimates and phenylpropanoids, 161 polyketides, 156 carbohydrates and 326 other derivatives, with a relative content of 24.746%, 12.035%, 25.739%, 14.267%, 10.792%, 3.813%, 2.366% and 6.242%, respectively. There were 35 compounds with a relative content of more than 0.5%, with a total relative content of 37.420%. The results of this study provide a reference for further exploration of bioactive secondary metabolites and functions of C. camelliae.
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