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人工老化与木素酸钠浸种对油松种子活力的影响
Effect of Artificial Aging and Sodium Lignate Immersion on seed Vigor of Pinus tabuliformis
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摘要: 为研究人工老化处理和木素酸钠浸种对油松种子活力及生理特性的影响，以油松种子为试验材料，采用高温高湿法对种子进行人工加速老化，测定不同老化处理时间（2、4、6、8、10 d，分别记为A2、A4、A6、A8、A10）下油松种子的发芽指标、MDA含量和SOD、POD、CAT活性，再使用3种不同浓度（25、50、100、200、400 mg/L）的木素酸钠（BCOL、BCL、BCMOL）处理未经老化与老化4 d的油松种子，分析油松种子的发芽势与发芽率差异。结果表明：随着老化处理时间的延长，油松种子的发芽率、发芽势和发芽指数显著下降，老化10 d种子发芽率降至25.67%。MDA含量大量积累，SOD、POD及CAT活性显著降低。用中等浓度BCOL（100 mg/L）、中等浓度BCL（50、100 mg/L）和中等浓度BCMOL（100 mg/L）对未经老化（A0）和老化4 d（A4）的油松种子处理，其发芽率较对照高出6%、4.66%、5.33%和10.33%、8.66%、10%。因此，人工老化油松种子超过4 d活力显著下降，同时种子内MDA积累、抗氧化酶活性降低。若需促进未经老化油松种子萌发，选用100 mg/L的BCOL与BCMOL、50 mg/L BCL浸种处理24 h效果较好；若需促进老化油松种子萌发，选用1种100 mg/L的木素酸钠进行浸种24 h即可。Abstract: In order to investigate the effects of artificial aging and Sodium Lignate immersion on seed vigor and Physiological Characteristics of Pinus tabuliformis seeds subjected to various aging durations(2, 4, 6, 8, 10 d, recorded as A2, A4, A6, A8, A10, respectively), artificial accelerated aging of seeds using high temperature and high humidity methods , using Pinus tabuliformis seeds as experimental materials. The research involved measuring germination indicators、malondialdehyde(MDA) content and the activities of superoxide dismutase(SOD), peroxidase(POD), and catalase(CAT) in seeds subjected to various aging durations. Additionally, three different concentrations(25, 50, 100, 200, and 400 mg/L) of sodium lignate(BCOL, BCL, and BCMOL) were applied to both non-aged seeds and seeds aged for 4 days. Analyzing differences in germination potential and germination rate of Pinus tabuliformis seeds. The results demonstrate that the germination potential、germination rate and germination index of Pinus tabuliformis seeds decrease progressively with extended aging periods. Seed germination decreased to 25.67% for 10 d of aging.Moreover, MDA content was heavily accumulated, while the activities of SOD, POD, and CAT activities were significantly reduced. Notably, Treatment of non-aged(A0) and Aged 4 d(A4) Pinus tabuliformis seeds with medium concentration of BCOL(100 mg/L), medium concentration of BCL(50, 100 mg/L) and medium concentration of BCMOL(100 mg/L) resulted in 6%, 4.66%, 5.33% and 10.33%, 8.66%, 10% higher germination rate than control.. Therefore, the vitality of artificially aged N upland pine seeds decreased significantly beyond 4 d, while MDA accumulation and antioxidant enzyme activities decreased in the seeds. If we need to promote the germination of un-aged seeds, 100 mg/L BCOL and BCMOL, 50 mg/L BCL seed immersion treatment for 24 h is more effective; if we need to promote the germination of aged seeds, we can choose a 100 mg/L sodium lignate seed immersion for 24 h. The results of the study can provide a reference basis for the conservation of Pinus tabuliformis. resources and aging seed germination.
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