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湖南木荷不同种源种实及苗期表型性状研究

Analysis and comprehensive evaluation of phenotypic traits of seeds and seedlings amomg different Schima superba Province in Hunan

  • 摘要: 选择12个种源的果实、种子、叶片及1年生苗木,测定果横径、果纵径、果柄长、叶长、叶宽、叶柄长、种子长、种子宽、地径和苗高等10个表型性状,利用方差分析、多重比较、相关性分析和聚类分析等方法,探究不同种源木荷的种实和苗期表型性状变异及其与环境因子的关系。结果表明:木荷8个种实与叶表型性状在种源间存在广泛变异,变异系数为8.9~25.82%,果横径、果柄长、叶宽、叶柄长存在显著性差异。不同种源木荷各表型性状与地理环境之间存在不同程度的相关性,果横径、叶长、叶宽、叶柄长、种子长和种子宽与北纬、pH存在显著负相关,苗高与东经呈显著负相关,与pH呈极显著正相关。苗期地径变幅4.23~5.71mm,苗高变幅30.10~45.00cm,桑植、龙山与茶陵、长沙木荷苗高呈显著性差异;苗期地径生长大于平均值百分比为1.28~16.6%,苗高生长大于平均值百分比为0.84~17.84%。通过聚类分析将12个木荷种源分为4大类型,第Ⅰ类包括金洞、通道、古丈和泸溪等4个湘西南产地,特征为果大、叶宽、生长快;第Ⅱ类包括靖州、南山公园、城步和资兴等4个湘东南产地,特征为果大,叶长、叶宽,生长较快;第Ⅲ类包括茶陵和长沙等湘东产地,特征为果小、生长一般;第Ⅳ类包括龙山和桑植等湘北产地,特征为果小、叶短、叶窄、种子小,生长快。果横径、果柄长、叶宽、叶柄长是木荷种源地理变异主要性状。东经、北纬、pH是影响种实表型性状的主要因素。通过综合评价,筛选出优良种源产区为桑植、龙山、南山公园和靖州。

     

    Abstract: Studying the diversity of seed and seedling phenotypic traits among different provenances of Schima superba in Hunan Province to provide a scientific basis for the collection, evaluation, and utilization of Schima superba germplasm resources. Variance analysis, multiple comparisons, correlation analysis, and cluster analysis were used to explore the variation in seed and seedling phenotypic traits among different provenances of Schima superba and their relationship with environmental factors by measuring fruit transverse diameter, fruit longitudinal diameter, fruit stem length, leaf length, leaf width, leaf stem length, seed length, seed width, ground diameter, and seedling height of fruits, seeds, and leaves, 1-year-old seedlings from 12 provenances. The results showed that: (1) There was widespread variation in eight seed and leaf phenotypic traits among different provenances of Schima superba, with coefficients of variation ranging 8.9%~25.82%. Significant differences were found in fruit transverse diameter, fruit stalk length, leaf width, and petiole length.(2)Various phenotypic traits of Schima superba from different provenances exhibit varying degrees of correlation with geographical environments. Fruit transverse diameter, leaf length, leaf width, petiole length, seed length, and seed width were significantly negatively correlated with northern latitude and pH. Seedling height was significantly negatively correlated with eastern longitude but extremely significantly positively correlated with pH. (3)During the seedling stage, the ground diameter ranged by 4.23~5.71 mm, and the seedling height ranged by 30.10~ 45.00 cm. Significant differences in seedling height were observed between Sangzhi, Longshan and Chaling, Changsha provenances of Schima superba. The percentage of ground diameter growth above the average during the seedling stage was 1.28% to 16.6%, and the percentage of seedling height growth above the average was 0.84%~17.84%.(4)The 12 provenances of Schima superba were divided into four types Variation of S. superba by cluster analysis,. The first category included four provenances in southwestern Hunan,including Jindong, Tongdao, Guzhang, and Luxi,which were characterized by large fruits, wide leaves, and fast growth.Category II included four provenances in southeastern Hunan,including Jingzhou, Nanshan Park, Chengbu, and Zixing, which were characterized by large fruits, long and wide leaves, and relatively fast growth.Category III included provenances in eastern Hunan such as Chaling and Changsha, characterized by small fruits and moderate growth.Category IV included provenances in northern Hunan such as Longshan and Sangzhi, characterized by small fruits, short and narrow leaves, small seeds, and fast growth. Fruit transverse diameter, fruit stalk length, leaf width, and petiole length were the main traits exhibiting geographical variation among the provenances of Schima superba.Eastern longitude, northern latitude, and pH were the main factors influencing the phenotypic traits of seeds and fruits. Through comprehensive evaluation, the excellent provenance regions were selected as Sangzhi, Longshan, Nanshan Park, and Jingzhou.

     

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