Abstract: Investigation and research on the distribution of amphibian diversity and their habitat selection can provide a scientific basis for amphibian conservation and habitat management. This study aims to investigate the amphibian diversity and habitat factors in Dianchi Lakeside wetland from April to November between 2021 and 2024. The differences in amphibian diversity among different habitats were analyzed using Shannon-Wiener diversity index, species richness and Correspondence Analysis. Principal component analysis and redundancy analysis were employed to analyze the selection and utilization of microhabitats by amphibians. The results indicate that Dianchi Lake harbors eight species of amphibians belonging to one order, distributed across four families, with estuarine wetlands exhibiting higher diversity compared to lake shore ponds or farmland swamps. Different species of amphibians may exhibit varying degrees of preference for specific combinations of microhabitats. Overall, the habitats with certain aquatic and terrestrial vegetation coverage, terrestrial vegetation coverage and relatively close distance from water source are important parameters for the habitat selection of amphibians. Strengthening the protection and management efforts in wetland habitats within the Dianchi Lake estuary is recommended. Additionally, planning for conservation areas with higher vegetation coverage located closer to water sources can effectively enhance the conservation efficiency of amphibian biodiversity resources in Dianchi Lake.