Abstract: A study on amphibian diversity and habitat factors was carried out in the Dianchi lakeside wetlands from April to November, spanning the years 2021 to 2024, to explore the distribution patterns of amphibians and their habitat preferences. The differences in amphibian diversity among different habitats were analyzed using Shannon-Wiener diversity index, species richness and correspondence analysis. Principal component analysis and redundancy analysis were employed to analyze the selection and utilization of microhabitats by amphibians. The results indicate that Dianchi Lake harbors 8 species of amphibians belonging to 1 order, distributed across 4 families, with diversity ranking estuary wetland>lakeside pond>farmland swamp. Different species of amphibians may exhibit varying degrees of preference for specific combinations of microhabitats. Overall, the habitats with certain aquatic and terrestrial vegetation coverage, terrestrial vegetation coverage and relatively close distance from water source are important parameters for the habitat selection of amphibians. Strengthening the protection and management efforts in wetland habitats within the Dianchi Lake estuary is recommended. Additionally, planning for conservation areas with higher vegetation coverage located closer to water sources can effectively enhance the conservation efficiency of amphibian biodiversity resources in Dianchi Lake.