Abstract: To explore the effects of coffee shell biochar, fulvic acid (FA) and N form on the growth and nutrient status of Pinus kesiya var. langbianensis seedlings for providing scientific theoretical basis for the directional cultivation of strong seedling. Using L₉(3⁴) orthogonal design, experiments were carried out with biochar supplemental level (0, 25, 50 g/L), FA concentration (0, 0.8, 1.6 g/L) and N form (no nitrogen, nitrate nitrogen, ammonium nitrogen). A total of 9 treatments were used. The effects of factor levels and their combinations on the growth and nutrient characteristics of P. kesiya var. langbianensis seedlings were analyzed. Coffee shell biochar, FA and N significantly promoted the seedlings growth (P < 0.05). High, ground diameterand biomass increased first and then decreased with the increase of biochar, and the growth of seedlings was the best at 25 g/L biochar. Biochar supplemental decreased N content of seedlings, but increased P and K content. With the increase of biochar, K content increased synchronously, but P content increased first and then decreased. FA application significantly increased height, biomass, N and P contents of seedling. N addition promoted the growth of seedlings and increased the contents of N and P. The growth and P content of seedlings treated with ammonium nitrogen were significantly higher than that of nitrate nitrogen, and ammonium nitrogen significantly increased the P content, while nitrate nitrogen had no significant effect on the P content (P≥0.05). Positive interaction effects between FA and N were observed on height and N content of seedling. Range of effect degree of each factor on seedling growth was coffee shell biochar > N > FA. Correlation analysis showed that seedling height, BD and biomass increased synergistically, and growth parameters were significantly correlated with P content (P < 0.01), that indicating P was a limiting element for seedling growth. Coffee shell biochar, FA and N promote seedling growth by enhancing the absorption of N, P and K. The optimal treatment is 25 g/L biochar and 0.8 g/L FA combined with ammonium nitrogen, and it is recommended to supplement P fertilizer appropriately during the production process.