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山新杨GATA基因家族成员鉴定及盐胁迫下基因表达分析

Identification and expression analysis of the GATA gene family in Populus davidiana × P. alba var. pyramidalis

  • 摘要: 为了揭示GATA转录因子在杨树盐胁迫应答中的作用,以山新杨为试验材料,通过转录组数据库搜索鉴定山新杨GATA基因家族,对PdaGATA转录因子家族基因进行了理化性质、系统发育、保守基序、启动子元件生物信息学分析,并通过RT-qPCR探讨它们在盐胁迫下不同组织中的表达模式。结果表明:山新杨中的24个GATA转录因子基因分4亚族,均含保守锌指结构域,同亚族基因结构高度同源。PdaGATAs与毛果杨和胡杨GATA成员同源性高,其启动子含多种激素和逆境响应元件;山新杨GATA基因呈现组织特异性表达模式。PdaGATA18在茎中表达最为丰富;PdaGATA1220在根系中表达显著;叶片中大多数PdaGATAs表现出较高表达水平。盐胁迫下,PdaGATAs表达谱发生显著变化,尤其是PdaGATA47131415显著上调,暗示这些基因在山新杨盐胁迫适应过程中发挥关键调控作用。

     

    Abstract: To investigate the role of GATA transcription factors in salt stress response, we characterized the PdaGATAs gene family in Populus davidiana × P. alba var. pyramidalis using transcriptome database analysis and bioinformatics tools. Bioinformatics analysis was used to examine the physicochemical properties, phylogeny, conserved motifs of PdaGATAs, and the expression patterns was explored by RT-qPCR in was different tissues under salt stress. The results show that 24 GATA transcription factor genes were identified in Populus davidiana × P. alba var. pyramidalis. Phylogenetic analysis and multiple sequence alignment revealed that PdaGATAs were classified into 4 subfamilies, all containing a conserved zinc finger domain with high structural homology within subfamilies. PdaGATAs have close relationships with GATA members from Populus trichocarpa and Populus euphratica, with promoters harboring hormone and stress-responsive elements. RT-qPCR results demonstrated tissue-specific expression patterns: PdaGATA18 showed highest expression in stems, PdaGATA1, 2, and 20 were significantly expressed in roots, and most PdaGATAs exhibited high expression levels in leaves. Under salt stress, expression profiles of PdaGATAs changed significantly, with notable upregulation of PdaGATA4, 7, 13, 14, and 15, suggesting their critical regulatory roles in salt stress adaptation in Populus davidiana × P. alba var. pyramidalis.

     

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