圆叶肿柄菊素A调控CYP724B1抑制番茄斑萎病毒NSm和 NSs基因复制研究

Tag A Regulation of CYP724B1 to Inhibite Tomato Spotted Wilt Orthotospovirus NSs Gene Replication

摘要: 圆叶肿柄菊素A（Tag A）能抑制番茄斑萎病毒（TSWV）侵染，利用实时荧光定量PCR检测发现经Tag A处理后，Nitab4.5_0007569g0010（CYP724B1）基因的复制量显著上调；利用病毒诱导的基因沉默（VIGS）技术，将CYP724B1基因沉默后进行预防实验处理。结果表明：基因CYP724B1沉默后，接种叶和系统叶中NSm、NSs基因的表达量与对照相比显著升高。将CYP724B1基因过表达后，接种叶和系统叶中NSm、NSs基因的表达量与对照相比显著降低。Tag A 通过调控CYP724B1基因抑制TSWV NSm、NSs基因的复制，进而抑制番茄斑萎病毒的侵染。

Abstract: Tagitinin A (Tag A) can inhibit the infection of Tomato spotted wilt orthotospovirus (TSWV). RT-qPCR was used to further verify the data and found that the expression of Nitab4.5_0007569g0010(CYP724B1) gene was differently significant increased after Tag A treatment. Using virus-induced gene silencing (VIGS) technology, the CYP724B1 gene was silenced, followed by preventive experimental treatment. The findings indicated that after CYP724B1 gene was overexpressed, the expression of the NSm and NSs genes was significantly decreased compared to the control in the inoculated and systemic leaves. These results indicate that Tag A could regulate the CYP724B1 gene to inhibit the expression of NSm and NSs to defense against TSWV infection.