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基于毛竹林地土壤高效溶磷细菌组合的复合菌肥构建及其促生机制研究

Construction of Compound Microbial Fertilizer Using High-Efficiency Phosphate-Solubilizing Bacterial combinations from Phyllostachys edulis Forest Soil and Its Growth-Promoting Mechanism

  • 摘要: 通过盆栽试验及不同载体培养接种比较试验,在毛竹苗生长季末期(T1)和第2年毛竹苗快速生长期末期(T2)取样进行各项指标测定,验证高效溶磷细菌菌株组合在毛竹幼苗生长过程中的功能效应,进一步构建菌株组合特异性载体,观测分析其对毛竹苗生长及土壤磷养分环境的影响。结果表明:细菌组合的冻干菌剂和液体菌剂对毛竹幼苗生长和养分积累起到显著促生作用,其中液体菌剂在T1时期促生效果好,根生物量和根系全氮含量分别较CK显著增加103%和15.65%;冻干菌剂在T2时期促生效果好,土壤全氮含量和有效磷含量分别较CK显著增加了16.11%和1105%,总生物量及全氮全磷含量较CK显著增加91.49%、76.32%和122.58%。花生壳与甘蔗渣1:1等体积混合适宜作为细菌组合的载体。添加该载体构建的固态溶磷细菌组合菌肥1 a后,土壤有效磷显著增加了408%;T1时期接种后毛竹苗根系、地上生物量较CK分别增加8.7%和41.9%;T2时期接种后毛竹苗根系、地上生物量较CK分别增加32.3%和746%。

     

    Abstract: At present, the available phosphorus elements are scarce globally, mostly inorganic phosphorus that cannot be used by plants, and the subtropical region of China is the main production area for Moso bamboo(Phyllostachys edulis). The primary soils in this region are red soil and brick-red soil, in which a large amount of phosphorus cannot be directly absorbed and utilized by plants. This process relies on phosphorus-solubilizing microorganisms for conversion and utilization. Therefore, in order to realize the rapid conversion and efficient utilization of phosphorus elements, this study used the pot experiment to sample the end of the growth season(T1) and the end of the rapid growth season(T2) to verify the functional effect of the efficient phosphorus-soluble microbial strain combination in the growth process of bamboo seedlings. Additionally, specific carriers for the microbial strains were constructed to observe and analyze their effects on seedling growth and the phosphorus nutrient environment in the soil. The results showed that both freeze-dried and liquid microbial agents from the synthetic microbial community significantly promoted seedling growth and nutrient accumulation. Among them, the liquid microbial agent had the best growth-promoting effect during the T1 period, with root biomass and total nitrogen content in the roots significantly increasing by 103% and 15.65%, respectively, compared to the control(CK). The freeze-dried microbial agent showed the best growth-promoting effect during the T2 period, with soil total nitrogen and available phosphorus content increasing significantly by 16.11% and 11.05 times, respectively. The total biomass, as well as total nitrogen and total phosphorus content, increased significantly by 91.49%, 76.32%, and 122.58%, respectively, compared to CK. Further analysis through different carrier inoculation comparative experiments showed that a 1:1 volume mixture of peanut shells and sugarcane bagasse was suitable as the carrier for the synthetic microbial community.One year after the application of the phosphate-solubilizing solid biofertilizer constructed with this carrier, the soil available phosphorus significantly increased by 408%. At the T1 stage, the root and aboveground biomass of Moso bamboo seedlings increased by 8.7% and 41.9%, respectively, compared to the control(CK). At the T2 stage, the root and aboveground biomass of Moso bamboo seedlings increased by 32.3% and 746%, respectively, compared to CK. The preliminary results demonstrate that the highly efficient phosphate-solubilizing bacterial combination has a significant effect on the growth of Moso bamboo seedlings, and a specific biofertilizer was developed, providing a reliable solution to address phosphorus deficiency during the growth of Moso bamboo seedlings.

     

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