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低温胁迫下外源糖处理对威宁短柱油茶苗激素含量、光合参数和叶片结构的影响

Effects of Exogenous Sugar Treatment on Hormone Levels, Photosynthetic Parameters, and Leaf Structure of Camellia weiningensis Seedlings under Low-Temperature Stress

  • 摘要: 以威宁短柱油茶盆栽幼苗为材料,设置常温处理和低温胁迫2种条件,分析不同外源糖喷施处理对油茶叶片激素含量、光合参数及叶片解剖结构的影响。结果表明:双因素分析显示温度和外源糖处理对赤霉素含量、部分光合参数和叶片解剖指标有显著影响。在低温胁迫下,与CK相比,外源半乳糖能显著提高吲哚乙酸的含量;低温抑制威宁短柱油茶净光合速率,但外源半乳糖、海藻糖和混合糖处理下净光合速率显著上升。此外,低温环境下外源半乳糖处理显著增加叶片上表皮厚度、栅栏组织厚度和组织紧密度。油茶叶片显微结构的变化与光合指标显著相关,而脱落酸与光合参数和叶片解剖指标的相关性不显著。因此,外源糖处理通过改善叶片解剖结构和光合性能,显著提升了威宁短柱油茶的耐低温能力,而外源半乳糖的处理效果要好于其他外源糖处理。

     

    Abstract: This study used potted Camellia weiningensis seedlings as experimental material to investigate the effects of different exogenous sugars(50 mM sucrose, 10 mM galactose, 10 mM trehalose, and mixed sugars) sprayed on hormone content, photosynthetic parameters, and leaf anatomical structure of leaves. The experiment was conducted under two conditions: normal temperature treatment(25 ℃/20 ℃, day/night) and low-temperature stress(10 ℃/5 ℃, day/night). Results showed that both temperature and exogenous sugar treatments significantly affected gibberellin(GA) content, photosynthetic parameters, and leaf anatomical indices. Under low-temperature stress, compared with the water-sprayed control(CK), exogenous galactose significantly increased the content of IAA. Low temperature inhibited the net photosynthetic rate(Pn) of C. weiningensis, but Pn values significantly increased under exogenous galactose, trehalose, and mixed sugar treatments. In addition, exogenous galactose treatments significantly increased the thickness of the upper epidermis, palisade tissue, and tissue compactness of the leaves. Correlation analysis indicated that changes in leaf microstructure were significantly correlated with photosynthetic parameters, while endogenous hormone ABA content showed no significant correlation with photosynthetic parameters or leaf anatomical indices. In conclusion, exogenous sugar treatments significantly enhanced the low-temperature tolerance of C. weiningensis by improving leaf anatomical structure and photosynthetic performance. The treatment with exogenous galactose is more effective than treatments with other exogenous sugars.

     

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