Abstract: To clarify the diversity and comprehensive evaluation of phenotypic trait, 101 single-flowered Clematis spp. varieties were used as experimental materials. Eleven quality traits and seven quantitative traits were performed on variation and diversity analysis. The findings showed that there were different in 11 quality traits with a variation coefficient of 14.06%–77.00% and a diversity index of 0.1971–1.6271. The variation coefficient of the midrib (77.00%) was the largest, indicating abundant variation types, high degree of variation, and substantial differences in the diversity index overall. The diversity index of 7 quantitative traits was 1.0274 to 1.4163 with rich genetic diversity, all of them were greater than 1.0. Most quantitative traits had highly significant correlation. Cluster analysis divided the 101 single-flowered Clematis spp. varieties into 4 categories by Class Ⅰ included 62 varieties with smaller flower diameters and average comprehensive performance of floral organ traits. Class Ⅱ gathered 21 varieties with medium-sized flower diameters. Class Ⅲ had only 1 variety characterized by the smallest flower diameter, calyx length, sepal aspect ratio, stamens number, and stamen length. Class IV had 7 varieties, mainly with larger flower types and excellent comprehensive performance of floral organs traits. Principal component analysis extracted 3 principal components with a cumulative contribution rate of 89.541%, which could represent comprehensively most of the information of single-flowered Clematis spp. phenotype data. Through comprehensive evaluation and ranking of single-flowered Clematis spp. phenotypic traits, the top 10 varieties were 'Thyrislund' 'Leinster' 'Kizashi' 'Dorothy Tolver' 'Olympia' 'Justa' 'Doctor Ruppel' 'Omoshiro' 'Snowdrift' and 'Luiza' with excellent comprehensive performance of floral organ traits.