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氮磷添加对香樟容器苗营养元素化学计量特征的影响
Effects of nitrogen and phosphorus addition on the stoichiometric characteristics of nitrogen, phosphorus, and potassium in Cinnamomum camphora container seedlings
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摘要: 为探讨氮磷添加对香樟容器苗营养元素(氮、磷、钾)化学计量特征的影响,以1年生香樟容器苗为试验材料,采用指数施肥法,对香樟容器苗进行低氮、中氮、高氮、磷、低氮+磷、中氮 + 磷、高氮 + 磷等施肥处理,以不施肥为CK。在幼苗停止生长后,测定其根、茎、叶生物量和营养元素(氮、磷、钾)的化学计量特征。结果表明:单施P处理下根的生物量达到最大值,为1.857 g/株,在N3下的茎、叶和总生物量显著大于其他处理;N3、N2P和N3P处理下香樟容器苗根氮含量显著高于其他处理,分别为23.83 mg/g、24.712 mg/g和24.876 mg/g;N2P为茎中氮含量的最高处理(15.035 mg/g);在叶中,除单施磷肥外,其他处理的氮含量均显著高于CK,其中N3和N2P处理的叶氮含量最高。单独施磷肥显著地提高了香樟容器苗各器官的磷含量,与CK呈显著差异(P<0.05),而单施氮肥和氮磷配施对苗木各器官磷含量的影响则不大。氮磷配施显著增加了香樟容器苗叶片的N/P,其中N3P处理叶片的N/P显著高于其他处理,为4.67;在根中N/P较高的处理是N2P和N3P,分别为6.18和6.16;N2P为茎中N/P最高的处理,为4.27。整体上看,N2P和N3P更有利于增加香樟容器苗根茎叶中N/P值。施肥对于香樟容器苗地下部分钾含量增加的影响不明显,主要集中在地上部分,尤其是叶片,且氮磷配施对于香樟容器苗叶片钾含量的促进作用最大。N2,N3和N2P处理下满足N/K>2.1且 K/P<3.4,香樟容器苗根部受到了钾的限制。因此,限制香樟容器苗的生长因素为氮限制,施氮或氮磷配施能显著增加苗木生物量,改变其各器官的N/P,缓解其氮限制情况。Abstract: : ObjectiveThe effect of nitrogen and phosphorus addition on the stoichiometric characteristics of nutrient elements (nitrogen, phosphorus, and potassium) in Cinnamomum camphora container seedlings was investigated to provide a theoretical and practical basis for further in-depth research on the nutritional characteristics and cultivation of plantation forests of C. camphora.Method The annual C. camphora container seedlings were used as the test materials, and the exponential fertilization method was used to apply fertilizer treatments including low nitrogen, medium nitrogen, high nitrogen, phosphorus, low nitrogen + phosphorus, medium nitrogen + phosphorus, high nitrogen + phosphorus, and no fertilizer was used as control (CK). After the seedlings stopped growing, the biomass and stoichiometric characteristics of the nutrient elements (nitrogen, phosphorus, potassium) in their roots, stems, and leaves were measured.Result The biomass of roots reached the highest amount under the single P application. The biomass of stem and leaves as well as the biomass of whole plant under N3 treatment were significantly higher that other treatments(P<0.05); The nitrogen mass fraction in roots of C. camphora container seedlings were 23.83 mg/g, 24.712 mg/g and 24.876 mg/g under N3, N2P and N3P treatments, respectively, which were significantly higher than other treatments. The nitrogen mass fraction in stem of C. camphora container seedlings was the highest under N2P treatment, which was 15.035 mg/g. In leaves, except for single application of phosphorus fertilizer, The nitrogen mass fraction of the other treatments were significantly (P<0.05)higher than those of the control group. The leaf nitrogen mass fractions of N3 and N2P treatments were the highest. Phosphorus fertilizer application alone significantly increased the phosphorus accumulation of each organ of C. camphora container seedlings, which was significantly different from that of the control (P<0.05), while single nitrogen application and combined nitrogen and phosphorus application had little effect on phosphorus accumulation of different organs. The combined application of nitrogen and phosphorus significantly increased the N/P of the leaves of C. camphora container seedlings, the N/P values of N3P treatments were the highest, which was 4.67. The highest N/P values in the roots were N2P and N3P treatments, which were 6.18 and 6.16, respectively; N2P treatment had the highest N/P value in stems, which was 4.27, and on the whole, N2P and N3P treatments were more conducive to increasing the N/P value in roots, stems and leaves of C. camphora container seedlings. The accumulation of potassium in the underground part of C. camphora container seedlings was not obviously affected by fertilization, however, the mass fraction of potassium in the aboveground part was significantly affected by the nitrogen and phosphorus addition, especially in the leaves; and the combined application of nitrogen and phosphorus had the greatest effect on the potassium mass fraction of C. camphora container seedlings. Under N2, N3 and N2P treatments, N/K and K/P meet the standard of N/K>2.1 and K/P<3.4 indicating that the roots of C. camphora container seedlings were limited by potassium.Conclusion Based on the stoichiometric ratio, it is proposed that under the conditions of this study, the factor restricting the growth of C. camphora container seedlings was nitrogen limitation. The single application of nitrogen fertilizer and combined application of nitrogen and phosphorus could significantly increase the biomass of C. camphora container seedlings, change the N/P ratio of various organs, and alleviate nitrogen limitation status.