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2种有机酸对镉胁迫下中国无忧花幼苗生理的影响

The Effects of Two Organic acids on physiology of Saraca dives Seedlings under Cadmium Stresses

  • 摘要: 以半年生中国无忧花幼苗为试材,采用双因素完全随机区组设计,设置5个镉水平和3个有机酸,分析不同处理下中国无忧花幼苗的生理特性,探究柠檬酸和苹果酸对不同镉胁迫下中国无忧花幼苗光合生理的影响。结果表明:不同镉浓度及有机酸处理对中国无忧花幼苗的光合及生理指标产生显著影响。镉胁迫显著降低Chl a/b比值,镉浓度高于50 mg/kg时,该比值呈先增后降趋势,加入有机酸Chl a/b比值提升;柠檬酸和苹果酸处理下最小值(Cd150CA4与Cd200MA4处理组)较同水平镉胁迫分别增加了13.36%和1.64%;PnGsTr随镉浓度升高而逐渐下降,有机酸的加入显著提高PnGsTr;各处理组的Ci随镉胁迫增强呈先降后升,在Cd200水平达到峰值r;镉胁迫下SS、SP含量呈先增后降,在Cd50处理组均达峰值,有机酸处理显著提升SS、SP含量,且苹果酸处理效果不及柠檬酸;Pro含量随镉浓度升高而增加,柠檬酸处理在Cd100时增幅最大;镉胁迫提升MDA含量,有机酸施加则显著降低;镉胁迫下SOD、POD和APX酶活性提高,施加有机酸后其活性进一步增强,尤其是柠檬酸处理组;隶属函数分析中,Cd100CA4处理组综合表现最佳,Cd50CA4次之。因此,在镉污染环境中,2种有机酸均能提升中国无忧花幼苗的光合生理特性和抗逆性,且柠檬酸效果优于苹果酸。

     

    Abstract: To investigate the effects of exogenous organic acids (citric acid and malic acid) on the photosynthetic physiology of Saraca dives seedlings under different cadmium stresses, a two-factor completely randomized block design was used to set up five cadmium levels and three organic acids to analyze the physiological characteristics of S. seedlings under different treatments, using six-month-old S. seedlings as test materials. The results showed that different cadmium concentrations and organic acid treatments had significant effects on the photosynthetic and physiological indexes of S. seedlings. Cadmium stress significantly reduced the Chl a/b ratio, which tended to increase and then decrease when the cadmium concentration was higher than 50 mg/kg, and then increased with the addition of organic acids, Cadmium stress significantly reduced the Chl a/b ratio, which tended to increase and then decrease at cadmium concentrations higher than 50 mg/kg, and the Chl a/b ratio was elevated by the addition of organic acids, with the minimum values(Cd150CA4 and Cd200MA4 treatment groups) under citric and malic acid treatments increasing by 13.36 and 1.64%, respectively, compared with the same level of cadmium stressl; Pn, Gs, and Tr decreased with the increase of cadmium concentration, and the addition of organic acids significantly increased Pn, Gs, and Tr; Ci in each treatment group showed a first decrease and then an increase with the enhancement of Cd stress, and reached a peak at the Cd200 level.; SS and SP content under Cd stress showed first increase and then decrease, in the Cd50 treatment group all reached the peak, organic acid treatment significantly enhanced SS, and SP content, and the malic acid treatment effect is not as good as citric acid; Pro content increased with cadmium concentration, citric acid treatment in the Cd100 when the maximum increase; cadmium stress to enhance the content of MDA, the imposition of organic acid was significantly reduced;The activities of SOD, POD and APX enzymes increased under Cd stress, and their activities were further enhanced by the application of organic acids, especially in the citric acid treatment group; in the analysis of the affiliation function, the Cd100CA4 treatment group had the best overall performance, followed by Cd50CA4.Therefore, in the Cd-contaminated environment, both organic acids were able to improve the photosynthetic physiological characteristics and stress tolerance of S. seedlings, and citric acid was more effective than malic acid.

     

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