Abstract: Using 1.5-year-old Zelkova schneideriana seedlings as experimental materials, this study investigated the effects of different trunk height treatments (2, 6, 10 cm) and paclobutrazol concentrations (0, 250,500,750,1000 mg/L) on dwarfing effects, providing valuable references for enhancing ornamental value management of Z. schneideriana. Results showed that under identical trunk height conditions, increased paclobutrazol concentration significantly inhibited plant height, shoot thickness, and internode spacing in seedlings, demonstrating notable dwarfing effects. Additionally, chlorophyll, soluble protein, soluble sugar, and SOD levels exhibited an initial increase followed by a decline with rising paclobutrazol concentration, with all treatment groups exceeding control values. MDA content, however, continued to rise with increasing paclobutrazol concentration. Considering the growth characteristics and ornamental value of Z. schneideriana seedlings, comprehensive analysis indicated that applying 500 mg/L paclobutrazol solution combined with 6 cm trunk height treatment achieved optimal dwarfing control while maintaining plant vitality.