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基于核磁共振技术探究红豆树种子的吸水特性

Water Absorption Characteristics of the Ormosia hosiei Seeds by Nuclear Magnetic Resonance Technique

  • 摘要: 为探究红豆树种子在吸水萌发过程中的水分空间及相态变化,通过核磁共振技术对吸水过程中的种子进行核磁成像观察其吸水位点和水分移动路径,结合横向弛豫时间T2探究种子在吸水萌发过程中的水分相态变化。结果表明:种孔是红豆树种子的主要吸水位点,水分由种孔进入后,一部分水分通过种皮和子叶间的缝隙以及种脐中的维管束向子叶末端移动,还有一部分水分被胚根吸收并向子叶中心扩散;弛豫图谱峰面积(S)与种子的水分质量(x, g)呈显著线性关系:S=8841.6x5440R2=0.9967;在种子吸水萌发过程中,各相态水分处于动态变化之中,束缚水和胞内水的含量先增加后减少,但比例整体上呈下降趋势;胞外自由水含量和比例均呈先快后缓的增加趋势,X10时比例升至69.7%,种子内水分流动性持续增强。核磁共振技术精准呈现了红豆树种子在吸水过程中的水分空间分布与相态变化,系统揭示了其吸水特性。

     

    Abstract: To investigate the spatial and phase changes of water in the Ormosia hosiei seeds during the water absorption and germination process, MRI technology was employed to observe their water absorption sites and the path of water movement during the water absorption process, and NMR technology was employed to get the transverse relaxation time T2 to explore the changes in water phase during the water absorption and germination process of the seeds. The results showed that the micropyle was the main water absorption site of the Ormosia hosiei seeds. After water entered through the micropyle, part of the water moved toward the end of the cotyledon through the gap between the seed coat and cotyledon as well as the vascular bundles in the hilum, while another part of the water was absorbed by the radicle and diffused toward the center of the cotyledon. The fitting results showed that the peak area (S) of the NMR relaxation spectrum had a significant linear relationship with the moisture mass (x, g) of the Ormosia hosiei seeds: S=8841.6x-5440, R2=0.9967. During the process of seed water absorption and germination, the various phases of water were in dynamic change. The content of bound water and intracellular water first increased and then decreased, but their proportion overall showed a declining trend. The content and proportion of extracellular free water both showed an increasing trend that was initially rapid and then slowed down. By X10, the proportion reached 69.7%, and the water mobility within the seeds continued to increase. Magnetic resonance technology accurately presented the spatial distribution and phase changes of water in the Ormosia hosiei seeds during the water absorption process, systematically revealing their water absorption characteristics.

     

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