Abstract: Collect the root zone soil of Camellia oleifera, prepare 0, 25, 50, 100, 200 g/L five concentrations of extraction solutions, respectively treat the seeds of Isatis tinctoria and Glycyrrhiza uralensis, and determine their germination rate, germination energy, root length, seedling height, SOD activity, POD activity, CAT activity, MDA content, Pro content, and chlorophyll content. Use the allelopathic effect index and the allelopathic comprehensive effect index to evaluate the intensity of the allelopathic effect. The results showed that at a low concentration of 25 g/L, the germination rates of I. tinctoria and G. uralensis significantly increased, with increases of 41.30% and 20.63%, respectively. The height of I. tinctoria seedlings increased by 10.37%, while the root length was inhibited by 17.76%. At the concentration of 25 g/L, the root length of G. uralensis was significantly inhibited by 11.87%. SOD activity in 2 Chinese medicinal herbs increased significantly. The POD activity of I. tinctoria first increased and then decreased, while the CAT activity was all inhibited. The MDA content first decreased and then increased with the increase in concentration. The changes in Pro content of 2 traditional Chinese medicinal herbs indicated their different osmotic adjustment strategies in response to allelopathic stress. The chlorophyll content increased with the increase in concentration. The allelopathic comprehensive effect index shows that for I. tinctoria, the SE value is 0.05 at concentrations of 25 g/L and 50 g/L, and turns to −0.02 at 100 g/L. The negative SE values of G. uralensis are not significant. The allelopathic effects of I. tinctoria and G. uralensis at all concentrations exhibit the patterns of promotion at low concentrations, inhibition or stability at high concentrations, and promotion at low concentrations, stability at high concentrations, or weak promotion, without showing strong inhibition. The allelopathic effect of the root zone soil extract of C. oleifera on I. tinctoria and G. uralensis is in a concentration-dependent pattern of low promotion and high stability. Low concentrations can safely promote germination and growth, while high concentrations will have a slight inhibitory effect.