Abstract: A study on the rooting culture of the Acacia crassicapa plantlets through photoautotrophic micropropagation was carried out.The results showed that the different culture media had significant effects on the rooting process of A.crassicapa, of which river sand was the optimal medium.The different nutrient solutions also had significant effects on the rooting of A.crassicapa in the photoautotrophic micropropagation experiment, of which the pure water without any nutritional components was the most effective.Different light intensity modes had significant effects on the rooting process on the above ground biomass and the underground biomass of A.crassicapa, of which Mode 2 was the optimal, i.e.the low light intensity within the range of 2500-3000 lx with the illumination duration of 12 h/d from the 0 to the 7th day; while the intermediate light intensity in the range of 4 000-5 000 lx with the illumination duration of 14 h/d between the 8th to the 10th day; and the moderate light intensity in the range of 6 000-8 000lx with the illumination duration of 14 h/d after the 10th day was applied separately in the different stages.