Abstract: The total genomic DNA was extracted from the dried leaves of Betula alnoides with CTAB, CTABSiO2, the nuclear separation, double times extraction, and modified CTAB methods, and the DNA obtained was analyzed by the means of agarose gel electrophoresis and RAPD analysis. The results showed that there was great difference in DNA yield from different extraction methods. The DNA yield by CTABSiO2 method was the lowest with 0.4-11.6μg/g. The DNA yield obtained by the nuclear separation and double times extraction methods was higher than the CTAB method, which could eliminate the glucose more efficiently. The DNA yield from the modified CTAB method was the highest, which was 171.2-364.8μg / g, the polymorphic patterns amplified by RAPD were the best with clear electrophoresis belts. It was concluded that the modified CTAB method was suitable to extract the total genomic DNA from dried leaves of Betula alnoides.