Abstract: 15 types of substrates with yellow soil as base, different proportions of calcium magnesium phosphate, superphosphate, and burned soil added as the basal fertilizers were applied for container seedling breeding experiment for Castanopsis fissa tree species. The experiment showed that the best result was obtained by treatment No. 12 (90% yellow soil + 10% burned soil), followed by treatment No.1 (98.8% yellow soil + 0.2% calcium magnesium phosphate), and treatment No. 6 (98.8% yellow soil + 0.2% superphosphate). The chlorophyll content of the seedling leaves bred with the latter two treatments was both lower than that of the control (pure yellow soil). When the burned soil was over 20%, or calcium magnesium phosphate was over 0.4%, or superphosphate was over 0.6% supplemented to the substrate, there seemed to be negative effect on the container seedlings of C. fissa.